BASIC NUCLEIC ACID STRUCTURES: FORM AND INTERACTION

基础核酸的构象和稳定性研究 结构及其与其他生物学上重要的 分子提出。 了解这些基本的 结构是我们理解核酸功能的关键， 蛋白质的合成，遗传器官的运作， 细胞过程的调节和化学试剂的作用方式 会导致突变和癌症 酶将被用来合成 尤其是较大核酸的令人感兴趣的寡聚组分。 的 这些低聚物的纯化将极大地通过高压 液相色谱法（HPLC）是我们开发的一种方法。 核磁 共振（NMR）实验将被用来研究结构 这些分子在水溶液中的细节。 这些实验将 与紫外线吸收的研究相关， 有序的核酸结构被破坏， 温度 这一信息关系到自然界的稳定性。 这些合成核酸的对应物。 进一步研究 合成寡核苷酸与药物、诱变剂和金属的相互作用 离子将导致关于诱变的分子基础的推断， 致癌作用以及提供合理药物设计的方法。

Investigations into the conformation and stability of basic nucleic acid structures and their interactions with other biologically important molecules are proposed. Knowledge of the properties of these basic structures is a key to our understanding the function of nucleic acids in the synthesis of proteins, the operation of the genetic apparatus, the regulation of cellular processes, and the mode of action of chemical agents that cause mutation and cancer. Enzymes will be used to synthesize especially interesting oligomeric components of larger nucleic acids. The purification of these oligomers will be greatly aided by a high pressure liquid chromatographic (HPLC) method we have developed. Nuclear magnetic resonance (nmr) experiments will be employed to investigate the structural details of these molecules in aqueous solution. These experiments will be correlated with studies of the absorption of ultraviolet light as the ordered nucleic acid structures are disrupted by increasing the temperature. This information bears upon the stability of the natural counterparts of these synthetic nucleic acids. Further studies on the interactions of synthetic oligonucleotides with drugs, mutagens, and metal ions will lead to inferences about the molecular basis of mutagenesis and Carcinogenesis as well as providing an approach to rational drug design.