REGULATION OF THE GAL GENES IN YEAST

这项研究的长期目标是了解 参与真核生物转录调控的机制 基因 为了达到这个目的，我们将分析转录的控制 半乳糖利用所需的酵母基因（GAL基因），其中 两个关键的调节基因产物（GAL 4和GAL 80）控制诱导 在转录水平上的GAL结构基因。 三 将进行相互关联的研究。 第一是聚焦 以鉴定启动子功能所需的DNA序列， 三个GAL结构基因的调控。 这涉及到分析 体外产生的突变对GAL-lacZ融合的影响。 第二 一系列研究包括构建过量生产克隆GAL的菌株 调节基因产物（GAL 4和GAL 80），然后纯化， 这两种蛋白质的特征。 第三条研究路线是 涉及开发用于转录酵母的转录系统， 通过RNA聚合酶II在体外表达。 在工作的各个阶段，我们计划 将体内进行的实验结果与 体外 希望这将使我们能够得出明确的结论 关于真核生物中转录控制的分子机制。

The long-range goal of this research is to understand the molecular mechanisms involved in the transcriptional regulation of a eukaryotic gene. To achieve this goal, we will analyze the control of transcription of yeast genes required for galactose utilization (the GAL genes), in which two key regulatory gene products (GAL4 and GAL80) control the inducibility of five GAL structural genes at the level of transcription. Three interrelated lines of research will be conducted. The first is focused towards identifying DNA sequences required for promoter function and proper regulation of three GAL structural genes. This involves analyzing the affects of mutations generated in vitro on GAL-lacZ fusions. The second line of research involves constructing strains which overproduce cloned GAL regulatory gene products (GAL4 and GAL80), followed by purification and characterization of these two proteins. The third line of research is directed towards developing a transcription system for transcribing yeast genes by RNA polymerase II in vitro. At all stages of the work, we plan to correlate the results of experiments performed in vivo with those performed in vitro. This will, hopefully, enable us to draw firm conclusions concerning molecular mechanisms of transcriptional control in eukaryotes.