ANTIBODY MODEL FOR RECOGNITION OF DNA HELICAL DIVERSITY

将用A、B和Z-DNA的代表性实例对动物进行免疫 和确定序列的碱基配对寡核苷酸。 所得多克隆 单克隆抗体将被测试它们区分 其中：不同家族的螺旋（A、B和Z）;各种螺旋 一个家庭的结构;和个人的各种地形特征 螺旋形 进一步的实验将测试抗体的能力 以检测插入到DNA中的独特序列依赖性螺旋构象， 线性或环状DNA分子，并影响动态平衡 不同的螺旋构象之间，可以假设由一个给定的 顺序 抗体也将用于探测相应的独特的 天然存在的DNA、染色质和细胞核中的螺旋区域。 这些 实验将提供检验假设的试剂和程序 在染色质中出现特定的局部构象特征 组织的DNA，并可能作为信号位点的过程中，调节 基因信息的转录或复制。

Animals will be immunized with representative examples of A, B and Z-DNA and base-paired oligonucleotides of defined sequence. Resulting polyclonal and monoclonal antibodies will be tested for their ability to distinguish among: helices of different families (A, B and Z); various helical structures of one family; and various topographical features of individual helical forms. Further experiments will test the ability of the antibodies to detect unique sequence-dependent helical conformation inserted into linear or circular DNA molecules and to affect the dynamic equilibrium between different helical conformations that can be assumed by a given sequence. Antibodies will also be used to probe for corresponding unique helical regions in naturally occurring DNA, chromatin and nuclei. These experiments will provide reagents and procedures for testing the hypothesis that specific local conformational features occur within the chromatin organization of DNA and may serve as signal sites in processes regulating transcription or replication of gene information.