ELECTRON TRANSPORT & ENERGY CONSERVATION IN MITOCHONDRIA

该提案概述了一系列调查，旨在提供 对理解电子转移和能量至关重要的信息 线粒体呼吸系统细胞色素Bc1区的转导 链条。该提议利用了各种生物物理和生化技术 提供结构和热力学信息的方法以及 调查组件之间功能交互的可能性。 结构信息将使用定向多层膜和 顺磁探测器接近了。氧化/还原滴定将 在各种条件下执行，以评估 各组成部分之间的协同性，并研究其原因和 光谱漂移的含义。这一领域很重要，因为 线粒体是动物细胞的主要能量来源，因为 相关的电子转移系统在许多方面都很重要 与能量转导没有直接联系的系统(如类固醇 激素合成，巨噬细胞过氧化氢的产生)，因为细菌 真菌的呼吸使用相似的呼吸链。

The proposal outlines a series of investigations which aim to provide information essential to the understanding of electron transfer and energy transduction in the cytochrome bc1 region of the mitochondrial respiratory chain. The proposal utilizes a variety of biophysical and biochemical approaches to provide structural and thermodynamic information and to investigate the possibility of functional interactions between components. Structural information will be obtained using oriented multilayer and paramagnetic probes approaches. Oxidation/reduction titrations will be performed under a variety of conditions to evaluate the possibility of cooperativity between various components and to study the causes and implications of spectral shifts. This area is important because mitochondria are the major source of energy for animal cells, because related electron transfer systems are important in a wide variety of systems not directly connected to energy transduction (e.g., steroid hormone synthesis, macrophage peroxide production) and because bacterial and fungal respiration utilize similar respiratory chains.