ELECTROPHORESIS OF HUMAN MALIGNANT HYPERTHERMIC MUSCLE

恶性高热(MH)是一种由 挥发性麻醉药和非去极化肌松药。其发病率 死亡率在1/15,000到1/50,000之间 大约30%。MH综合征的特征是代谢性酸中毒， 心动过速，肌肉僵硬，体温明显升高。这个 MH的术前诊断很困难，因为它需要大量的 肌肉活检，致力于MH诊断的实验室和生理学家 学习。那些有家族史的人可以进行筛查 肌肉活检对MH的影响，但对肌肉的现有检测也携带 相当程度的不确定性。这项提议旨在延长我们的 先前观察到存在新的低分子量蛋白质 在MH肌肉中。我们打算获得更多的肌肉样本 来自证实了MH发作的患者。肌肉蛋白质会 进行电泳法，分离和鉴定额外的蛋白质。这个 额外的MH蛋白将通过密度扫描进行定量 凝胶电泳法及其与MH易感性的相关性 额外制造的MH蛋白的数量。

Malignant hyperthermia (MH) is a pharmacogenetic disorder provoked by volatile anesthetics and non-depolarizing muscle relaxants. Its incidence is between 1 in 15,000 and 1 in 50,000 anesthetics with a mortality of approximately 30%. The MH syndrome is characterized by metabolic acidosis, tachycardia, muscle rigidity and markedly elevated temperature. The preoperative diagnosis of MH is difficult because it requires a large muscle biopsy, a laboratory and physiologist dedicated to MH diagnostic studies. Those individuals with a previous family history can be screened for MH by muscle biopsy but the available tests of the muscle also carry a fair degree of uncertainty. This proposal is intended to extend our previous observations that novel low molecular weight proteins are present in MH muscle. We intend to obtain a greater number of muscle specimens from patients who have had verified MH episodes. The muscle proteins will be electrophoresed and the extra proteins isolated and characterized. The extra MH proteins will be quantitated by densitometric scans of the electrophoretic gels and a correlation between MH susceptibility and the amount of extra MH proteins made.