PARACOCCUS DENITRIFICANS IRON TRANSPORT APPARATUS

本计划将集中在分离的一个组成部分的副球菌 铁的运输工具。 该计划的主要目的是 选择性和不可逆地标记微生物的铁载体表面 受体，以促进其分离和最终的结构 澄清。 希望这种受体的分离将提供 更好地了解生物体如何利用其 铁载体，parabactin，在铁的同化。 该计划将 分为两部分：1）帕拉菌素的设计和合成， 受体标记和2）将对羟基苯甲酸菌素标记固定到副球菌上 嗜水气单胞菌parabactin受体。 初步证据显示， 下面的文字强烈表明，该计划具有很高的指数， 成功 我们已经展示了我们在合成 相关系统以及我们处理相关 微生物操作。

This program will focus on the isolation of a component of the Paracoccus denitrificans iron transport apparatus. The major aim of the program is to selectively and irreversibly label the microorganism's siderophore surface receptor in order to facilitate its isolation and eventual structural elucidation. The hope is that the isolation of this receptor will provide us with a better understanding of how the organism utilizes its siderophore, parabactin, in the assimilation of iron. The program will be divided into two segments: 1) the design and synthesis of a parabactin receptor label and 2) the fixing of the parabactin label to the Paracoccus denitrificans parabactin receptor. The preliminary evidence presented in the text to follow strongly suggests that the program has a high index of success. We have already demonstrated our expertise in synthesis of related systems as well as our ability to handle the associated microbiological manipulations.