PROPERTIES OF MICROTUBULE PROTEIN

本研究的目的是了解 微管蛋白的自组装和微管形成的控制， 解聚 在这些研究中，我们正在研究微管蛋白的多态性， 通过确定一些变量对自组装的影响 形成的微管的原丝数和复合物的形成 带状和片状的原丝。 我们也在调查 6S微管蛋白自组装过程中存在的中间体。 的角色 巯基和二硫键的微管蛋白正在检查。 这些将是 用诸如氟代二硝基苯的试剂特别改性， N-乙基马来酰亚胺、丹磺酰氯、丹磺氮丙啶和2-巯基乙醇。 将确定这些试剂对微管蛋白物理结构的影响 用差光谱法、荧光法和圆二色光谱法 问题研究 紧密结合的金属离子的功能也正在研究中。 Mn、Zn、Co交换结合态Mg对物理结构的影响 的微管蛋白和自组装反应进行了研究。

The purpose of the research is to gain an understanding of the mechanism of self-assembly of tubulin and the control of microtubule formation and depolymerization. In these studies we are examining the polymorphism of tubulin self-assembly by determining the effects of a number of variables on the protofilament number of formed microtubules and on the formation of complex ribbons and sheets of protofilaments. We are also investigating the types of intermediates which are present during self-assembly of 6S tubulin. The roles of sulfhydryl and disulfide bonds in tubulin are being examined. These will be modified specifically with agents such as fluorodinitrobenzene, N-ethylmaleimide, dansyl chloride, dansylaziridine, and 2-mercaptoethanol. Effects on the physical structure of tubulin by these agents will be determined by difference spectroscopy, fluorimetric and circular dichroism spectroscopy studies. The function of the tightly bound metal ion is also being examined. The effects of exchanging bound Mg with Mn, Zn, and Co on the physical structure of tubulin and the self-assembly reaction will be investigated.