MECHANISMS IN RESPIRATION

我们建议对细胞色素进行详细的物理化学研究 c1aa3和Rieske铁硫蛋白从极 嗜热需氧菌，Thermus thermophilus。 初步研究 这些蛋白质的功能成分是相同的 从线粒体中获得的类似蛋白质。 我们的研究需要 细菌代谢特异性引入探针原子的优势 我们的实验将带来新的 了解这些蛋白质的活性位点结构以及它们如何 功能 计划在本赠款期内开展的工作是提供信息， 以及用于构建“最小组件”呼吸系统的材料 系统 这表明从极端嗜热菌中获得的蛋白质， 由于它们对变性的抗性，是理想的受试者， 详细检查呼吸机制。

We propose to carry out detailed physical-chemical studies of cytochrome c1aa3 and the Rieske iron-sulfur protein isolated from the extremely thermophilic, aerobic bacterium, Thermus thermophilus. Preliminary studies have shown that the functional components of these proteins are identical to analogous proteins obtained from mitochondria. Our studies take advantage of bacterial metabolism to specifically introduce probe atoms into the molecules of interest, and our experiments will lead to new knowledge of the active-site structures of these proteins and how they function. The work proposed for this grant period is planned to supply information and materials from which to construct a "minimum component" respiratory system. It is suggested that proteins obtained from extreme thermophiles, because of their resistance to denaturation, are ideal subjects for detailed examination of mechanisms in respiration.