GLYCOPROTEIN ALTERATIONS IN DIFFERENTIATION

这项研究的总体目标是定义生化变化 出现在细胞表面糖蛋白的寡糖中 在T淋巴细胞分化和功能表达过程中 自然杀伤细胞。N-连接的寡糖的表达将是 从整个细胞以及特定的糖蛋白Thy1中进行检测 和Ly5/T200，表现出分化相关的改变。这个 研究的最优先事项是低聚糖的特性 含有聚乳糖胺结构，因为它们已经被认识到 与许多生物学上的分化和成熟有关 系统，最显著的是造血系统。要使用的单元格类型 在目前的研究中将包括T淋巴系和自然杀伤(NK) 处于不同分化和成熟阶段的细胞。克隆T细胞和NK细胞 具有不同细胞溶解或辅助功能的细胞也将被研究。 细胞表面糖蛋白的生化特征将是 使用系列凝集素层析，聚乳糖胺特异性 曼陀罗和番茄凝集素，并与抗II亲和力结合 柱子。细胞表面多聚乳糖的表达将与 小鼠个体发育和成熟过程中低聚糖的变化 造血系统。低聚糖的作用将在 抗Thy1抗体诱导T细胞增殖和NK-肿瘤细胞黏附 随后的裂解。特异寡糖的鉴定 具有特定生物学特性的细胞的离散亚群的改变 角色将是我们理解差异化的重要一步， 发展转型。

The overall goal of this study is to define the biochemical alterations that occur in oligosaccharides of cell surface glycoproteins during differentiation and during the functional expression of T lymphocytes and natural killer cells. The expression of N-linked oligosaccharides will be examined from whole cells as well those from specific glycoproteins, Thy1 and Ly5/T200, which exhibit differentiation related alterations. The highest priority of the study is the characterization of oligosaccharides containing polylactosamine structures because they have been recognized to be associated with differentiation and maturation in a number of biological systems, most notably the hematopoietic system. The cell types to be used in the current study will encompass T lymphoid and natural killer (NK) cells at various stages of differentiation and maturation. Cloned T and NK cells with different cytolytic or helper functions will also be studied. The biochemical characterization of cell surface glycoproteins will be accomplished using serial lectin chromatography, polylactosamine specific Datura stramonium and tomato lectins, and binding to anti-Ii affinity columns. Cell surface polylactosamine expression will be related to oligosaccharide alterations observed during ontogeny and maturation in the hematopoietic system. The role of oligosaccharides will be studied in anti-Thy1 induced T cell proliferation and NK-tumor cell adhesion and subsequent lysis. The identification of specific oligosaccharide alterations in discrete subsets of cells that have specific biological roles will be an important step in our understanding of differentiation, development and transformation.