GENE EXPRESSION IN CELL ORGANELLES

总体目标是阐明控制和控制的一些机制 裸藻叶绿体中依赖DNA的RNA转录调控 好的。具体项目包括：(1)选址和DNA 叶绿体转录单位的序列分析及其可能的调控 区域，(2)光期间特定转录本的变化的量化 诱导叶绿体发育，(3)在体外系统中鉴定 参与选择性RNA转录的成分。在未来一年，我们计划 以下项目：(1)继续部分DNA序列分析 核糖体RNA转录单位和选定的mRNA编码区，包括 核酮糖-1，5-二磷酸羧基酶大亚基基因，(2) 完成叶绿体RNA聚合酶亚基结构的研究，(3) 通过解决和分析初级转录本和加工途径 某些特定转录单位的Northern杂交分析 DNA序列数据可用，(4)继续一般的作图程序， 裸藻叶绿体基因组的克隆和基因定位。

The overall objective is to elucidate some of the mechanisms for the control and regulation of DNA-dependent RNA transcription in chloroplasts of Euglena gracilis. Specific projects include the following: (1) Location and DNA sequence analysis of chloroplast transcription units and putative regulatory regions, (2) Quantitation of changes in specific transcripts during light induced chloroplast development, (3) Characterizing in an in vitro system the components involved in selective RNA transcription. In the coming year we plan the following projects: (1) Continue DNA sequence analysis of portions of the ribosomal RNA transcription units, and selected mRNA coding regions, including the gene for the large subunit of ribulose-1,5 -bis phosphate carboxylase, (2) Complete a study of the subunit structure of chloroplast RNA polymerase, (3) Analyze the primary transcripts and processing pathways via solution and Northern hybridization analysis for some specific transcription units for which DNA sequence data are available, (4) Continue a general program of mapping, cloning, and gene localization for the Euglena chloroplast genome.