MOLECULAR TOPOGRAPHY OF PHOTOMORPHOGENIC RECEPTOR

在植物中有两种主要类型的细胞光反应和某些 光感受器光敏色素介导的单细胞藻类。 这些光反应的分子机制在很大程度上是未知的。这个 因此，拟议研究的长期目标是了解 光敏色素在分子水平的作用机制。我们的具体目标 建议的研究有两个方面：(1)研究分子 光敏色素的可逆光活化对其形貌的影响 对光敏色素的活性形式不起作用，以及(2)表征 活性光敏色素形式(PFR)与其相互作用方式 潜在/假定的“受体”和分子拓扑学模型 探测器。由于基因调控作用和光敏转导 由光敏色素介导的过程可能会显示出一个有用的对比 和/或与激素基因调控和感觉转导相似 (例如，视觉)在动物身上，拟议的研究与健康有关 了解调节和感觉生物学的基本原理。

There are two major types of cellular photoresponses in plants and certain unicellular algae mediated by the photoreceptor, phytochrome. The molecular mechanisms of these photoresponses are largely unknown. The long term objective of the proposed study, therefore, is to understand the mechanisms of phytochrome action at the molecular level. Our specific aims of the proposed research are two-fold: (1) to investigate the molecular topography of phytochrome as affected by the reversible photoactivation of the inactive to the active form of phytochrome, and (2) to characterize the mode of interactions between the active phytochrome form (Pfr) and its potential/putative "receptors" and models in terms of molecular topographic probes. Since the gene-regulatory role and the photosensory transduction processes mediated by phytochrome are likely to reveal a useful contrast and/or similarity to the hormonal gene regulation and sensory transduction (e.g., vision) in animals, the proposed study is health-related in understanding the basic principles of regulatory and sensory biology.