BIOMEMBRANE MECHANICS, SURFACE AFFINITY AND ADHESION

血细胞的变形性是重要的决定因素 在微循环中的流量分布，也调节细胞 聚集、凝集和吞噬相互作用。 的 本研究的目的是量化时间依赖性 白色血细胞的机械性能， 血细胞变形和细胞间的化学吸引 粘合和粘合剂触点分离的力学。 细胞聚集的自由能势能定量了细胞聚集的可能性。 细胞表面“识别”的初始阶段， 在去除异常细胞和物质的因素 循环，病原体感染细胞， 循环白色和肿瘤细胞的边缘化。 强度 附着力（由分离所需的力表示） 接触）确定细胞聚集体的活力， 在循环中受到剪切力的破坏。 具体目标旨在解决以下一般问题： 问题：被动语态的共性和多样性是什么？ 白色血细胞之间的流变学特性？ 有 血液吞噬细胞的区别特征 有哪些 代表主动机械应力的固有特性 以及这些特性是如何与 细胞内的化学反应 什么是亲和力 通过特异性凝集作用促进粘附 反应？ 每个横桥的“断裂”能量是多少， 稳定粘合剂接触对抗环境力量？ 冲击力在凝集反应中如何发挥作用？ 独特的显微操作技术被用来执行 单细胞变形性和细胞间的微力学试验 粘连 观察结果用视频显微镜记录下来 系统 配体-受体的表面结合和分布 复合物是用视频增强激光测量的， 荧光测量系统，耦合到显微镜系统。

Deformability properties of blood cells are essential determinants of flow distribution in the microcirculation and also modulate cell aggregation, agglutionation, and phagocytic interactions. The objectives of this research are to quantitate the time-dependent mechanical properties of white blood cells, the energetics of blood cell deformation and chemical attraction in cell-cell adhesion, and the mechanics of separation of adhesive contacts. The free energy potential for cell aggregation quantitates the initial stage of cell-surface "recognition" which is an important factor in the removal of aberrant cells and material from the circulation, infection of cells by pathogens, arrest and margination of circulating white and tumour cells. The strength of adhesion (represented by the forces required to separate contacts) determines the viability of cell aggregates when exposed to disruption by shear forces in the circulation. The specific aims are designed to address the following general questions: what is the commonality and diversity of passive rheological properties amongst white blood cells? Are there distinguishing features between blood phagocytes? What are the intrinsic properties that represent active mechanical stresses generated by phagocytes and how can these properties be related to chemical reactions inside cells? What is the affinity that acts at long range to promote adhesion via specific agglutination reactions? What is the "fracture" energy per crossbridge that stabilizes adhesive contacts in oppostion to environmental forces? How do impingement forces play a role in agglutination reactions? Unique micromanipulation techniques are used to perform micromechanical tests of single cell deformability and cell-cell adhesion. The observations are recorded with a video microscope system. Surface binding and distribution of ligand-receptor complexes are measured with a video intensified, laser fluorometry system, coupled to the microscope system.