STRUCTURE AND FUNCTION OF THY-1

Thy-1是一种主要在T细胞上表达的膜糖蛋白 淋巴细胞和神经细胞。 由于其结构 与免疫球蛋白结构域同源，已经提出， Thy-1可能作为T细胞上的受体发挥作用。 事实上， 研究表明，针对Thy-1的抗体 可以刺激T细胞增殖并产生淋巴因子。 在 与其他参与调节细胞膜的膜蛋白不同， 在免疫系统中，Thy-1似乎锚定在膜上， 通过糖磷脂，该糖磷脂取代了31个氨基酸的片段， 新合成的Thy-1的羧基末端。 突变体和 嵌合Thy-1基因将被构建并用于瞬时表达。 和稳定的转染系统，以研究 这种转化和糖脂锚在膜中的作用 “运输”和T细胞活化。

Thy-1 is a membrane glycoprotein expressed predominantly on T lymphoyctes and neuronal cells. Because of its structural homology to immunoglobulin domains, it has been proposed that Thy-1 may function as a receptor on T cells. Indeed, several studies have demonstrated that antibodies directed against Thy-1 can stimulate T cells to proliferate and produce lymphokines. In contrast to other membrane proteins involved in regulation of the immune system, Thy-1 appears to be anchored to the membrane via a glycophospholipid which replaces a 31 amino acid segment at the carboxyl terminus of newly synthesized Thy-1. Mutant and chimeric Thy-1 genes will be constructed and used in transient and stable transfection systems to investigate the mechanism of this conversion and the role of the glycolipid anchor in membrane "trafficking" and T cell activation.