BIOENERGETICS OF CYTOCHROME B-C1 COMPLEX

该项目的总体目标是更好地了解 调节线粒体生物合成的因子。 虽然线粒体具有 合成不到其总蛋白质的10%的能力，8-10 在线粒体内产生的疏水蛋白质对于 形成功能性呼吸链和ATP合成复合物。 这 项目建议调查控制合成的机制， 线粒体蛋白质在两个不同的细胞内位点。 具体 在两个不同的转录和翻译水平上的可能控制 将通过使用从酵母线粒体分离的多核糖体进行研究。 线粒体信使RNA将被表征，这将是线粒体的性质。 新生链存在于多聚核糖体上。 结合这些研究， 细胞色素B的组装，一种线粒体蛋白质合成的建议产物， 进入呼吸链的复合物III将通过使用特定的 突变体以及纯化酶的抗体。 这些拟议 实验应该有助于阐明线粒体膜是如何组装的。

The overall objective of this project is to gain a greater understanding of the factors which regulate mitochondrial biogenesis. Although mitochondria have the ability to synthesize less than 10% of their total proteins, the 8-10 hydrophobic proteins made within the mitochondria are essential for the formation of a functional respiratory chain and ATP synthesizing complex. This project proposes to investigate the mechanisms wwhich control the synthesis of mitochondrial proteins at the two different intracellular sites. Specifically possible controls at the two different transcriptional and translational level will be investigated by the use of polysomes isolated from yeast mitochondria. Mitochondrial messenger RNAs will be characterized as will be the nature of the nascent chains present on the polysomes. In conjunction with these studies, the assembly of cytochrome b, a proposed product of mitochondrial protein synthesis, into complex III of the respiratory chain will be studied by use of specific mutants as well as antibodies to the purified enzyme. These proposed experiments should help elucidate how the mitochondrial membrane is assembled.