BIOANALYTICAL MICROSEPARATIONS, DETECTION AND PERFUSION

该提案涉及的发展和表征 主要基于微柱分离的生物分析方法 技术. 我们感兴趣的系统本质上是神经化学的。 这是一个以方法开发为导向的提案。 我们计划调查 碳膜电极作为柱上电化学检测器的应用 用于微柱液体分离方案。 这个探测器非常 敏感，应适用于任何大小的列和应用程序， 毛细管区带电泳是可行的。 毛细管带的使用 涉及神经化学感兴趣化合物的电泳图 计划使用激光荧光和电化学检测。 最后，我们建议使用电渗流来获得极小的 并将这些样品“注入”毛细管 电泳柱 类似的方案将用于进行体内 大鼠灌流实验。 这些实验可以用 并排的毛细管被拉长到只有几微米的直径。

This proposal concerns the development and characterization of bioanalytical methods based primarily upon microcolumn separation techniques. The systems we are interested in are neurochemical in nature. This is a methods development oriented proposal. We plan to investigate the use of a carbon film electrode as an on-column electrochemical detector for microcolumn liquid separation schemes. This detector is extremely sensitive, should be applicable to any size column and application to capillary zone electrophoresis is feasible. The use of capillary zone electrophoresis for schemes involving neurochemically interesting compounds is planned with both laser fluorescence and electrochemical detection. Lastly, we propose to use electroosmotic flow to acquire extremely small volume samples and to "inject" these samples onto the capillary electrophoresis column. A similar scheme will be used to carry out in vivo perfusion experiments on rats. These experiments can be carried out with side-by-side capillaries drawn out to only a few microns in diamter.