ELECTRICAL CONTROL OF SPERM EGG FUSION

这项有关“精卵融合电控”的建议，涉及 三个问题的调查： 1.受精是如何引起卵子膜电位变化的？ 2.卵子的膜电位如何调节精卵融合？ 3.精子膜是否参与精卵融合的调节？ 该方法学涉及细胞内的电生理技术 微电极和贴片电极记录。1)我们将分析 在蛙卵受精时出现的电导增加， 测量整个电池和单通道电流，并检查 膜的添加和去除以及钙在调节细胞生长中的作用 电导。2)我们将使用跨物种受精来确定 无论是精子还是卵子贡献了潜在的敏感成分 参与精子和卵子的融合。我们将启动一个项目来研究 通过开发程序来实现电压敏感融合的机制 分离精子的融合区域并将其与卵子融合。3) 我们将测量豚鼠不同位置的膜电位。 猪精子，然后修改潜力，同时检查对 导致精子和卵子融合的事件。 我们的长远目标是促进我们对 精卵融合的调控和我们对精卵融合的普遍认识 受精。

This proposal on "Electrical Control of Sperm-Egg Fusion" involves an investigation of three questions: 1. How does fertilization cause the egg's membrane potential to change? 2. How does the egg's membrane potential regulate sperm-egg fusion? 3. Does the sperm's membrane participate in regulation of sperm-egg fusion? The methodology involves electrophysiological techniques for intracellular microelectrode and patch electrode recording. 1) We will analyze the conductance increase that occurs at fertilization in the frog egg, measuring the whole cell and single-channel currents, and examining the roles of membrane addition and removal and of calcium in regulating the conductance. 2) We will use cross-species fertilization to determine whether the sperm or the egg contributes the potential-sensitive component involved in sperm-egg fusion. We will begin a project to examine the mechanism of voltage-sensitive fusion by developing procedures for isolating the fusogenic region of the sperm and fusing it with eggs. 3) We will measure the membrane potential at various positions on the guinea pig sperm, and then modify the potential while examining the effects on events leading to sperm-egg fusion. The long-term objectives are to contribute to our understanding of the regulation of sperm-egg fusion and to our general knowledge of fertilization.