HORMONAL REGULATION OF LUTEAL CELL DIFFERENTIATION

这项研究的总体目标是研究内分泌。 细胞分化和孕酮合成的调节 大鼠和兔的黄体。具体地说，生理作用 卵泡膜黄体细胞以及雌激素和黄体生成素在卵泡发育中的作用 将强调黄体颗粒细胞的分化。两个基本要素 将使用本实验室最近开发的方法。第一, 黄体颗粒细胞或黄体整体移植 将卵泡制成切除垂体的Fischer 344大鼠，并在 兔子。第二，来自兔的颗粒黄体细胞将在 单层培养。将检验以下假设：1)雄激素 (或其芳香化衍生物)和催乳素是增加 大鼠颗粒黄体素的类固醇合成能力和进一步分化 细胞在排卵后第二周；2)膜的重要作用 叶黄素细胞参与黄体雌激素的合成，这是 促黄体生成素刺激(大鼠)；3)大鼠小的类固醇生成细胞群 兔黄体来源于卵泡膜细胞；4) 促黄体生成素在兔黄体中的作用是刺激募集 或小黄体细胞分化，怀疑源于卵泡膜； 5)黄体颗粒细胞分化及孕酮合成 黄体生成素或雌二醇都能刺激培养；类固醇激素的生成 雌二醇的作用是通过3‘5’-环磷酸腺苷(3‘5’-cAMP)介导的。这些 预计研究将导致对相互作用的新理解 黄体细胞群和荷尔蒙之间的关系 主治黄体的正常功能和退化。

The overall objective of this research is to investigate the endocrine regulation of cell differentiation and of progesterone synthesis in the corpus luteum of the rat and rabbit. Specifically, the physiological role of the theca lutein cell and the role of estrogen and of LH in the differentiation of the granulosa lutein cell will be emphasized. Two basic approaches, recently developed in this laboratory, will be used. First, transplants of luteinizing granulosa cells or of luteinizing whole follicles will be made into hypophysectomized Fischer 344 rats and in rabbits. Second, granulosa lutein cells from rabbits will be studied in monolayer cultures. The following hypotheses will be tested: 1) androgen (or its aromatized derivative) and prolactin are necessary for increased steroidogenic capacity and further differentiation of rat granulosa lutein cells in the second week after ovulation; 2) an important role of the theca lutein cell is its participation in luteal estrogen synthesis, which is stimulated by LH (rat); 3) the small steroidogenic cell population in rat and rabbit corpora lutea is derived from thecal cells of the follicle; 4) the role of LH in the rabbit corpus luteum is to stimulate the recruitment or differentiation of small luteal cells, suspected to be of thecal origin; 5) differentiation and progesterone synthesis by granulosa lutein cells in culture can be stimulated either by LH or by estradiol; the steroidogenic effect of estradiol is mediated by 3'5' - cyclic AMP (rabbit). These studies are expected to lead to new understanding of the interactions between cell populations in the corpus luteum and of the hormones that govern the normal function and regression of the corpus luteum.