CONTROL AND INTEGRATION OF GNRH NEURAL PATHWAYS

灵长类中含促性腺激素释放激素(GnRH)的神经元 下丘脑内侧基底核(MBH)及其与其他脑区的关系 脑内多肽能神经元、儿茶酚胺能神经元和雌激素合成神经元 对猴和大鼠的MBH和正中隆起(ME)进行了观察。1. 狒狒MBH内GnRH神经元与其腹侧的明显接触 下丘脑束(VHT)由免疫细胞化学(ICC)识别。 用光学显微镜(LM)观察振镜切片的染色和 然后用电子显微镜(EM)检查。电紧性的证据 利用逆行荧光法获得MBH神经元之间的(缝隙)连接 茎切片恒河猴的黄色(LY)标记。莱伊 使用PAP对荧光染料偶联细胞进行GnRH ICC染色 技术，并由EM检查缝隙连接。GAP的其他证据 连接是用冷冻断裂的灵长类VHT寻找的。2)单元格 确定VHT和结节漏斗通路的起始体 逆行真蓝(TB)标记在茎切片恒河猴中的应用 猴子。结核荧光细胞经ICC染色测定GnRH 组件。TB的结果与LY的结果进行了比较，细胞 身体分布已绘制地图。3)芳香酶(ARO)在大鼠体内的定位 并用ICC检测特定的ARO酶亚基 在LM级别。特别注意内侧视前区， 大鼠性二形核(SDN-POA)的定位 MBH是ARO活性最高的两个脑区。如果大鼠SDN-POA 是ARO阳性的，我们将ICC染色为ARO来鉴定等效性 狒狒体内的SDN-POA。4)多巴胺受体之间的关系 (DA)、b-内啡肽(完)和催乳素(PRL)，以及ME神经末梢 对它们的发生进行了调查。使用ME突触体、DA、END、PRL 受体用ICC和GaRIg G-胶体金进行鉴定。内容 用ICC测定GnRH、END或酪氨酸的标记突触体数 羟色胺(TH，DA神经元的标志物)和PAP技术。5) GnRH神经元与其他据信影响GnRH的神经元之间的相互作用 在大鼠和狒狒的MBH中检测到分泌物。双标签ICC是 针对GnRH执行，然后针对END、ARO或TH执行ICC。互动 经LM鉴定后，经EM检查。这是关于GnRH的信息 神经系统、相互连接和传入输入将增强我们的 灵长类动物生殖神经控制的知识和理解， 包括人类。

Gonadotropin releasing hormone (GnRH) containing neurons in the primate medial basal hypothalamus (MBH), and their relationship to other peptidergic, catecholaminergic, and estrogen-synthesizing neurons in the MBH and median eminence (ME) of the monkey and rat are investigated. 1. Apparent contacts between GnRH neurons in the baboon MBH and its ventral hypothalamic tract (VHT) are identified by immunocytochemical (ICC) staining on vibratome sections, observed with light microscopy (LM), and then examined with electron microscopy (EM). Evidence for electrotonic (gap) junctions between MBH neurons is obtained using retrograde Lucifer Yellow (LY) labelling in the stalk-sectioned rhesus monkey. LY fluorescent, dye-coupled cells are ICC stained for GnRH using the PAP technique, and examined by EM for gap junctions. Other evidence for gap junctions is sought using freeze fracture of the primate VHT. 2) Cell bodies of origin of the VHT and tuberoinfundibular pathways are identified with retrograde True Blue (TB) labelling in the stalksectioned rhesus monkey. TB fluorescent cells are stained by ICC to determine the GnRH component. Results with TB are compared to those with LY, and the cell body distribution mapped. 3) Localization of aromatase (ARO) in the rat and baboon brain is determined using ICC for specific ARO enzyme subunits at the LM level. Special attention is given to the medial preoptic area, the location of the sexually dimorphic nucleus (SDN-POA) in rats, and the MBH, the two brain regions with highest ARO activity. If the rat SDN-POA is ARO-positive, we will ICC stain for ARO to identify the equivalent of the SDN-POA in the baboon. 4) Relationships between receptors for dopamine (DA), b-endorphin (END), and prolactin (PRL), and the ME nerve terminals on which they occur is investigated. Using ME synaptosomes, DA, END, PRL receptors are identified with ICC and GaRIgG-colloidal gold. The content of labelled synaptosomes is determined using ICC for GnRH, END, or tyrosine hydroxylasle (TH, as a marker for DA neurons) and the PAP technique. 5) Interactions between GnRH neurons and others belived to affect GnRH secretion are examined in the rat and baboon MBH. Dual-label ICC is performed for GnRH followed by ICC for END, ARO, or TH. Interactions identified by LM are examined by EM. This information regarding GnRH neuronal systems, interconnections, and afferent input will enhance our knowledge and understanding of neural control of reproduction in primates, including humans.