GENE REGULATION IN TERATOCARCINOMA AND EMBRYONIC CELLS

我们建议进行以下方面的调查： 1.研究调控组织转录的决定因素 纤溶酶原激活物(t-PA)基因在三种实验小鼠系统中的应用 A)不同分化阶段的畸胎癌干细胞；b) 可变表达t-PA基因的组织提取物；以及c)生长 卵母细胞。 2.探讨组织型纤溶酶原激活剂(t-PA)在小鼠胚胎发育和 正常生理学：a)检查t-PA的空间和时间方面 原位分析在胚胎和成体中的mRNA表达； 与目标1结合，确定t-PA启动子的哪些区域是 通过分析转基因小鼠有助于组织特异性表达 包含t-PA启动子/报告基因的构建；以及C)研究 T-PA基因的一个或两个拷贝的破坏对小鼠的影响 利用同源重组技术。 3.鉴定一种新型纤溶酶原激活剂(PA) 土星蛾科毛虫。

We propose the following areas of investigation: 1. To study the determinants that regulate the transcription of the tissue plasminogen activator (t-PA) gene using three experimental murine systems: a) teratocarcinoma stem cells in various stages of differentiation; b) extracts of tissues that variably express the t-PA gene; and c) growing oocytes. 2. To investigate the role of t-PA in mouse embryonic development and normal physiology by: a) examining spatial and temporal aspects of t-PA mRNA expression in the embryo and adult by in situ analysis; b) in conjunction with Aim 1, determining which regions of the t-PA promoter are instrumental in tissue-specific expression by analyzing transgenic mice containing t-PA promoter/reporter gene constructs; and C) investigating the consequences of disruption of one or both copies of the t-PA gene in mice using the techniques of homologous recombination. 3. To characterize a new type of plasminogen activator (PA) produced by a Saturnidae moth caterpillar.