NEUROENDOCRINE REGULATION OF OVARIAN CYCLICITY

季节性繁殖中卵巢周期性的神经内分泌调节 物种是这个项目的主题。 将进行研究， 识别与光周期、松果体、下丘脑和 类固醇激素对LH脉冲性分泌调节及对雌二醇的反应 负反馈在母羊身上。 将特别强调 关于这些信号的模式化对产生 应答 下丘脑促性腺激素释放激素和松果体褪黑激素信号的模式 将特别感兴趣，并将使用便携式背包进行研究 输液系统 在下丘脑中立体定位放置刀切口， GnRH在绵羊脑神经元中的免疫细胞化学定位将 用于检查光周期和类固醇的神经处理 调节脉冲LH分泌的信号。 放射免疫测定将 用于测量血清LH、FSH、GnRH、褪黑激素、雌二醇和 孕酮 预计这些发现将带来新的见解 关于卵巢周期性和季节性繁殖， 可逆转的生育能力

The neuroendocrine regulation of ovarian cyclicity in a seasonally breeding species is the theme of this project. Studies will be performed to identify signals pertinent to photoperiodic, pineal, hypothalamic and steroidal regulation of pulsatile LH secretion and response to estradiol negative feedback in the female sheep. Particularl emphasis will be placed on the importance of patterning of these signals to generation of responses. The patterns of hypothalamic GnRH and pineal melatonin signals will be of special interest and will be studied using a portable back-pack infusion system. Stereotaxically placed knife-cuts in the hypothalamus and immunocytochemical localization of GnRH in neurons of the sheep brain will be used to examine the neural processing of photoperiodic and steroidal signals which regulate pulsatile LH secretion. Radioimmunoassays will be used to measure serum LH, FSH, GnRH, melatonin, estradiol and progesterone. It is expected that these findings will lead to new insight concerning ovarian cyclicity and seasonal breeding, an natural process of reversable fertility.