FETAL SKIN BIOLOGY

结构、生物化学和功能的多学科研究 计划在开发过程中对人类皮肤的特性进行研究。 例正常 胚胎和胎儿皮肤将用于建立组织学和 表皮、真皮和羊水的超微结构特征 细胞群体在发展的进行性阶段。 这些数据将 作为所有其他项目的共同标准; 来自用于形态学研究的相同胚胎和胎仔的皮肤 将用于：定量真皮-表皮的半桥粒 表皮的连接、生化测定和免疫定位 丝聚蛋白和角蛋白，特异性生化鉴定 表皮、皮下脂肪和羊水中的脂质种类， 通过表皮转运的评价，生化鉴定 以及真皮中主要间质胶原的免疫定位， 以及皮肤糖胺聚糖的鉴定和定量， 真皮胶原酶的合成和活性的评价，在不同的 发展阶段。 胎儿皮肤活检和尸检样本 遗传性皮肤病也将进行形态学研究， 改变和许多生化程序，我们的组织 数量允许。 这些项目的数据有望扩大我们的 了解正常人类皮肤生物学在发展过程中，作为 对照，基线数据对于评估 有严重遗传性皮肤病风险胎儿的皮肤结构和特性 的珠被疾病，扩大了产前诊断的潜力， 皮肤疾病和扩展我们的理解的自然历史， 遗传性皮肤病进入产前阶段。

A multidisciplinary study of the structural, biochemical and functional properties of human skin during development is planned. Samples of normal embryonic and fetal skin will be used to establish histologic and ultrastructural characteristics of the epidermis, dermis and amniotic fluid cell populations at progressive stages of development. These data will serve as the common denominator of all other projects; parallel samples of skin from the same embryos and fetuses used for the morphologic studies will be used for: quantitation of hemidesmosomes at the dermal-epidermal junction, biochemical assay and immunolocalization of the epidermal proteins filaggrin and keratin, biochemical identification of specific classes of lipids in the epidermis, subcutaneous fat and amniotic fluid, evaluation of transport across the epidermis, biochemical identification and immunolocalization of the major interstitial collagens in the dermis, and identification and quantitation of dermal glycosaminoglycans, evaluation of synthesis and activity of dermal collagenase, at various stage of development. Biopsy and autopsy samples of skin from fetuses with inherited cutaneous disorders will also be studied for morphologic alterations and by as many of the biochemical procedures our tissue quantity allows. The data from these projects are expected to expand our understanding of normal human skin biology during development, serve as control, baseline data essential for evaluation of abnormalities in cutaneous structure and properties in fetuses at risk for severe inherited disorders of the integument, expand tee potential for prenatal diagnosis of skin disorders and extend our understanding of the natural history of genetic skin diseases into the antenatal period.