DEFECTS IN MULLERIAN DUCT REGESSION

在狗的两种遗传性综合征中，苗勒管持续存在于 睾丸组织的存在：XX性反转和持续性苗勒氏症 导管综合征(PMDS)。该项目的目标是确定 在这些动物模型中是否缺乏苗勒管回归 人类的中间性是缺乏穆勒抑制的结果 物质(管理信息系统)。如果是这样的话，这将是第一个被定义的遗传缺陷 迄今知之甚少的管理信息系统。 通过已知的育种将产生XX性反转和经前综合症的狗 承运人。将比较受影响幼崽的性腺的管理信息系统的活性 利用分级器官从正常雄性后代的性腺中提取 管理信息系统的培养试验。将对管理信息系统检测的结果进行检查 关于性腺结构，特别是支持细胞的形态， 使用光学和电子显微镜。

In two hereditary syndromes of the dog, Mullerian ducts persist in the presence of testicular tissue: XX sex reversal and Persistent Mullerian Duct Syndrome (PMDS). The objective of this project is to determine whether the lack of Mullerian duct regression in these animal models of human intersexuality is the result of a deficiency in Mullerian Inhibiting Substance (MIS). If so, these will be the first defined genetic defects in the as yet poorly understood MIS system. Dogs with XX sex reversal and PMDS will be produced by breeding known carriers. The MIS activity of gonads from affected pups will be compared with that of gonads from normal male littermates using a graded organ culture assay for MIS. Results from MIS assays will be examined with respect to gonadal structure, particularly the morphology of Sertoli cells, using light and electron microscopy.