A CELL LINEAGE STUDY OF AVIAN SOMITE FORMATION

本申请中提出的工作旨在增加我们对以下方面的了解： 脊椎动物体节模式是如何产生和维持的。 具体地 建议使用胚胎培养和嫁接技术， 鸡胚和鸡胚之间的不分节体节中胚层的 日本鹌鹑。 这些交流将以这样一种方式进行， 由宿主胚胎指定的体节模式与 体节模式，将已形成的嫁接中胚层，如果它有 被留在原地。 通过使用组织学分析和时间推移 通过电影显微摄影，将确定宿主胚胎是否有能力 调控嫁接体节的形成数量和时间。 的信息 从这项研究中得出的结论有望帮助我们最终了解 脊柱的形态发生和畸形发生。

The work proposed in this application is intended to increase our knowledge of how the veterbrate somite pattern is generated and maintained. Specifically it is proposed to use embryo culture and grafting techniques to exchange portions of the unsegmented somite mesoderm between embryos of the chicken and the Japanese quail. These exchanges will be carried out in such a way as to create disharmonies between the somite pattern specified by the host embryo and the somite pattern that would have been formed by the grafted mesoderm if it had been left in place. Through the use of histological analysis and time-lapse cinemicrography, it will be determined if the host embryos have the ability to regulate the number and time of formation of the graft somites. The information derived from this study is expected to aid in our eventual understanding of the morphogenesis and the teratogenesis of the vertebral column.