SYNTHESIS OF MITOCHONDRIAL PROTEIN

这个项目的总体目标是更好地了解 调控线粒体生物发生的因素。虽然线粒体有 合成不到总蛋白质10%的能力，8-10 线粒体中产生的疏水蛋白质对 形成功能呼吸链和三磷酸腺苷合成复合体。这 该项目建议研究控制合成的机制。 线粒体蛋白在两个不同的细胞内位置。特指 在转录和翻译两个不同水平上的可能控制 将通过使用从酵母线粒体中分离出的多聚体进行研究。 线粒体信使RNA的特征将是 新生的链存在于多聚体上。与这些研究相结合， 线粒体蛋白质合成产物细胞色素b的组装， 进入呼吸链的复合体III将通过使用特定的 突变体以及对纯化酶的抗体。这些建议 实验应该有助于阐明线粒体膜是如何组装的。

The overall objective of this project is to gain a greater understanding of the factors which regulate mitochondrial biogenesis. Although mitochondria have the ability to synthesize less than 10% of their total proteins, the 8-10 hydrophobic proteins made within the mitochondria are essential for the formation of a functional respiratory chain and ATP synthesizing complex. This project proposes to investigate the mechanisms wwhich control the synthesis of mitochondrial proteins at the two different intracellular sites. Specifically possible controls at the two different transcriptional and translational level will be investigated by the use of polysomes isolated from yeast mitochondria. Mitochondrial messenger RNAs will be characterized as will be the nature of the nascent chains present on the polysomes. In conjunction with these studies, the assembly of cytochrome b, a proposed product of mitochondrial protein synthesis, into complex III of the respiratory chain will be studied by use of specific mutants as well as antibodies to the purified enzyme. These proposed experiments should help elucidate how the mitochondrial membrane is assembled.