DEVELOPMENTAL CONTROL OF THE DIAPHRAGM AND UPPER AIRWAYS

这项研究的目的是为了更好地了解 协调上呼吸道肌肉激活的机制 与出生后的横膈膜的发育过程相吻合。功能障碍 该系统导致早产儿中发现的阻塞性呼吸暂停发作， 患有阻塞性睡眠呼吸暂停的婴儿和成人。这些实验 旨在测量膜性能的变化， 控制横膈膜和上呼吸道的呼吸运动神经元。 这项建议的具体目的是确定内在的作用， 这些运动神经元的特性在塑造他们的呼吸 开发过程中的放电行为。更具体地说， 运动神经元的特性控制其神经元的行为？的 控制这种行为的细胞和离子机制的演变 将在出生后的发育过程中进行研究。通过这些研究，我们 希望确定在处理过程中是否有任何不同， 这两个运动池之间的成熟，如果是这样，什么是相对的 达到成年放电特征的顺序？ 这些呼吸系统的主动和被动膜特性 将使用细胞内记录研究运动神经元， 标签技术。这些特性背后的离子机制 将使用单电极电流和电压钳来描绘， 在体内和体外哺乳动物制剂中。体外脊髓 脊髓和脑干，因为将开发，以研究 在发育过程中特定离子电流的改变。

The goal of this research is to gain a better understanding of the mechanisms which coordinate the activation of the upper airway muscles with that of the diaphragm during postnatal development. Dysfunction in this system results in episodes of obstructive apneas found in premature infants and adults with obstructive sleep apnea. These experiments are designed to measure the changes in the membrane properties of the respiratory motoneurons controlling the diaphragm and upper airways. The specific aim of this proposal is to determine the role the intrinsic properties of these motoneurons play in shaping their respiratory discharge behavior during development. More specifically, do intrinsic properties of the motoneuron control its neuronal behavior? The evolution of cellular and ionic mechanisms controlling this behavior will be studied during postnatal development. From these studies, we hope to ascertain if there is any difference in the process of maturation between these two motor pools and if so, what is the relative sequence of attaining their adult discharge characteristics? The active and passive membrane properties of these respiratory motoneurons will be investigated using intracellular recording and labeling techniques. The ionic mechanisms underlying these properties will be delineated using single electrode current and voltage clamp in both in vivo and in vitro mammalian preparations. An in vitro spinal cord and brainstem since will be developed in order to study the alteration in specific ionic currents during development.