EICOSANOIDS AND PREGNANT HUMAN MYOMETRIUM

二十烷基类化合物在子宫肌层的调节中起着重要作用 宫缩。虽然有些二十烷类化合物会刺激子宫收缩， 其他人可以抑制它们。这两组人之间复杂的相互作用 二十碳酸类化合物可能是子宫音调的决定因素。受体 结合是二十烷类化合物作用的先决条件，但这些 受体也存在于怀孕的子宫肌层中，以及它们是否改变 在怀孕和分娩期间还没有调查过。二十烷酸类化合物作用 不仅需要它们的受体，还需要它们的产物。这两个都是 很可能受怀孕期间存在的其他荷尔蒙的调节。 最后，二十烷类化合物与受体结合后的作用机制(S)如下 不清楚。利用生化、分子和细胞相结合的方法 生物技术，我们将研究PGF、PGF2在子宫肌层中的变化 α、PGI2和LTC4受体、环氧合酶、5-脂氧合酶、前列腺素E_2、前列环素 妊娠、早产和足月的异构酶和PGF2α合酶 劳力。此外，对妊娠人子宫肌层二十烷类化合物的调节 合成酶、二十烷类化合物的产生和二十烷类受体 孕期激素及二十烷类化合物的作用机制(S) 行动也将受到调查。几乎没有一个方面是 本提案中讨论的二十烷类化合物的生产和结合已经 在妊娠人子宫肌层中进行了研究。预计数据将提前 我们对二十烷类化合物作用和机制的认识(S) 有助于更好地理解S临产的机理 足月和早产。更好的理解可以提供更有效的方法 推迟早产和足月选择性引产。这个 推迟或预防早产可能会节省很大一部分时间 现在用于新生儿重症监护的医疗保健资金。

Eicosanoids play a fundamental role in the regulation of myometrial contractions. While some eicosanoids stimulate uterine contractions, others can inhibit them. The complex interplay between these two groups of eicosanoids is a likely determinant of the tone of the uterus. Receptor binding is a prerequisite for eicosanoids action, but whether these receptors are also present in pregnant myometrium and whether they change during pregnancy and labor has not been investigated. Eicosanoid action require not only their receptors but also their production. Both of these may very well be regulated by other hormones present during pregnancy. Finally, the mechanism(s) of eicosanoids action after receptor binding are unclear. Using the combination of biochemical, molecular and cell biological techniques, we will investigate myometrial changes of PGF, PGF2 alpha, PGI2 and LTC4 receptors, cyclooxygenase, 5-lipoxygenase, PGE2, PGI2 isomerases and PGF2 alpha synthase during pregnancy, preterm and term labor. In addition, regulation of pregnant human myometrial eicosanoid synthesizing enzymes, eicosanoids production and eicosanoid receptors by hormones present during pregnancy and the mechanism(s) of eicosanoids actions will also be investigated. Virtually none of the aspects of eicosanoids productions and binding discussed in this proposal have been studied in the pregnant human myometrium. The data is expected to advance our knowledge of the role and mechanism(s) of eicosanoids actions which should help to better understand mechanism(s) of initiation of labor at term and preterm. Better understanding can suggest more effective methods of delaying preterm labor and elective induction of labor at term. The delay or prevention of preterm labor can potentially save a large segment of the health care dollars now used for intensive neonatal care.