CARBOHYDRATE AND FSH SPECIFICITY DETERMINATION

本研究的总体目标是了解结构-功能 促性腺激素的相互关系。促性腺激素，黄体生成素 激素(黄体生成素)、卵泡刺激素(FSH)和绒毛膜 促性腺激素(CG)在生殖调控中起着核心作用。 促性腺激素被用来治疗不孕症，以确定 排卵，并表明人类怀孕。此外，人类CG 在某些癌症中被用作肿瘤标志物。生物力学的实验模型 促性腺激素的作用是基于这些激素在大鼠体内的作用。一个 在这些研究中，卵泡刺激素的常见替代品是马绒毛膜 促性腺激素。心电的初级结构牢固地确立了它是一种 黄体生成素/卵泡刺激素是糖蛋白激素家族的成员，但已知有 FSH在许多生物检测中都有活性。马的黄体生成素具有相同的蛋白质 结构与心电信号，也有促卵泡激素活性。两个版本的比较 心电刺激大鼠颗粒细胞的心电活动和促性腺激素释放激素 揭示了它们对这些荷尔蒙的不同反应。自从Elh 而心电图只在碳水化合物部分上有所不同，这表明 这些细胞正在检测碳水化合物结构上的这些差异。 具体目标#1是描述阿尔法亚单位的作用 寡糖对颗粒细胞反应的刺激作用。一系列 混合荷尔蒙由ELH、CG和 EFSH亚基将被准备好，它们的刺激能力 我们将比较心电刺激和DES刺激的颗粒细胞中类固醇的生成。 这项实验将证明阿尔法的差异 亚基低聚糖是导致反应差异的原因 这些颗粒细胞对每种激素的作用。ELH与心电结合的研究进展 将对大鼠卵巢的冰冻切片进行鉴定。具体目标2 目的是研究寡糖在靶细胞激活中的作用。 从ELH、CG、EFSH和EFSH的α亚基中纯化的寡糖 将脑下垂体游离α进行比较，以确定 每种激素上的α亚基低聚糖种群。这个 寡糖将在#年的颗粒细胞检测系统中进行测试。 以确定它们是否会干扰对FSH的反应 刺激。

The overall goal of this research is to understand the structure-function relationships of the gonadotropins. The gonadotropins, luteinizing hormone (LH), follicle-stimulating hormone (FSH), and chorionic gonadotropin (CG), play a central role in the regulation of reproduction. Gonadotropins are used to treat infertility, to determine the time of ovulation, and to indicate pregnancy in humans. In addition, human CG is used as a tumor marker in certain cancers. Experimental models of gonadotropin action are based on action of these hormones in rats. A frequent substitute for FSH in these studies has been equine chorionic gonadotropin. The primary structure of eCG firmly establishes it as an LH/CG member of the glycoprotein hormone family, yet it is known to have FSH activity in many bioassays. Equine LH shares identical protein structure with eCG and also has FSH activity. Comparison of the activities of eCG and ELH in stimulating eCG-primed rat granulosa cells has revealed differences in their response to these hormones. Since eLH and eCG differ only in their carbohydrate moieties, this suggests that the cells are detecting these differences in carbohydrate structure. Specific aim #1 is to characterize the role of the alpha subunit oligosaccharides in stimulation of granulosa cell response. A series of hybrid hormones consisting of all possible combinations of eLH, eCG, and EFSH subunits will be prepared and their abilities to stimulate steroidogenesis in eCG- and DES-primed granulosa cells will be compared. This experiment will demonstrate whether or not differences in alpha subunit oligosaccharides are responsible for the differences in response to each hormone by these granulosa cells. The binding of eLH and eCG to frozen sections of rat ovaries will be characterized. Specific aim #2 is to investigate the role of oligosaccharides in target cell activation. Oligosaccharides purified from alpha subunits of eLH, eCG, EFSH, and pituitary free-alpha will be compared to determine the differences in populations of alpha subunit oligosaccharides on each hormone. The oligosaccharides will be tested in the granulosa cell assay systems in order to determine if they can interfere with the response to FSH stimulation.