OZONE EFFECT ON RESPIRATORY TRACT MUCUS AND PERMEABILITY

将对人体的呼吸道粘膜进行检查 非侵入性地使用无线电气雾剂来确定 环境污染物臭氧对呼吸道粘液纤毛功能的影响。 重复暴露在0.2和0.4ppm臭氧中将进行研究，以 确定是否发生了粘膜功能的适应，以及 气道功能是否与主观适应有关 症状与臭氧对人体健康的影响有关。 粘液纤毛功能。功能异常的吸烟者将被 在纵向研究中进行评估，以确定粘液纤毛 功能，已知这些受试者在习惯化过程中会抑郁 对于香烟，受到臭氧暴露的影响；在后续行动中，在 戒烟6个月，对臭氧的粘液纤毛反应 将重新评估暴露情况。臭氧对环境影响的修饰 将用利多卡因研究粘膜生理学 气雾剂，用于麻醉对臭氧敏感的呼吸道感受器，以及 用抗坏血酸(口服)，以减少组织介质的释放 在臭氧吸入过程中。最后，臭氧暴露对 呼吸气溶胶的沉积分数将被评估为3 2、4和6微米直径的颗粒尺寸代表 粗粒态颗粒物污染。这些粒子将被 有无线电标记，因此将提供有关 肺内的区域性沉积。我们的技术利用伽马 相机成像和舱内臭氧曝露。这个 获得的信息将支持我们的假设，粘液 膜生理学是肺的主要决定因素 对刺激性刺激的反应，并更好地定义 环境污染物在呼吸道诱导或进展中的作用 异常现象。

In man the respiratory mucous membranes will be investigated non-invasively with radio-aerosols to determine the effects of ambient pollutant, ozone, on airway mucociliary function. Repetitive exposure to 0.2 and 0.4 PPM ozone will be studied to determine if adaptation of mucus membrane function occurs, and whether the adaptation of airway function and subjective symptoms are causally related to the effects of ozone on mucociliary function. Smokers, with abnormal function, will be evaluated in longitudinal study to determine if mucociliary function, known to be depressed in these subjects while habituated to cigarettes, is influenced by ozone exposure; at follow-up, after 6 months of smoking cessation, mucociliary response to ozone exposure will be re-evaluated. Modification of ozone effects on mucous membrane physiology will be studied with lidocaine aerosol, to anesthetize airway receptors sensitive to ozone, and with ascorbic acid (orally), to reduce tissue mediators released during ozone inhalation. Lastly, the effect of ozone exposure on the deposition fraction of respired aerosol will be evaluated for 3 particle sizes, 2, 4 and 6 micron m diameters, representatives of coarse mode particulate pollution. These particles will be radiolabeled and thus will provide additional information on regional deposition within the lung. Our technique utilizes gamma camera imaging and chamber exposures to ozone. The information obtained will support our hypothesis that mucus membrane physiology is a primary determinant of the lung response to irritant stimuli, and better define the role of environmental pollutants in the induction or progression of airway abnormalities.