CLONING OF THE HMG CO-A SYNTHASE GENE

这个项目的长期目标是扩大我们对 胆固醇生物合成中酶的遗传调控。这类研究 它们的科学意义既来自一些不同寻常的机会 在这个系统中研究基因表达的转录调控和 利用这些研究得出的见解来增加我们的 对其发病机制及最终化疗的认识 动脉粥样硬化性心血管疾病。具体地说，这个项目 描述了针对该酶克隆人类基因的研究。 DNA技术合成3-羟基-3-甲基戊二酰辅酶A 介导性基因转移。建议的方法提供了一个工具，而不是 仅用于克隆基因的信息元件，但用于克隆 调控序列也参与了它的表达。我们还提出了 为了确定在这些克隆的DNA序列中的什么位置 序列在于，构造两个泛函上的限制图 基因及其调控成分，最后鉴定和 决定调控表达的特定DNA序列的活性 这种基因的基因。

The long range goal of this project is to enlarge our understanding of the genetic regulation of enzymes in cholesterol biosysnthesis. Such studies derive their scientific significance both from some unusual opportunities in this system to study transcriptional control of gene expession and the utilization of insights derived from these studies to increase our understanding of the pathogenesis and eventual chemotherapy of atherosclerotic cardiovascular disease. Specifically, this project describes studies directed at cloning the human gene for the enzyme 3-hydroxy-3-methyglutaryl coenzyme A synthases by the technique of DNA mediated gene transfer. The methodology proposed provides a vehicle not only for cloning informational elements of the gene, but for cloning regulatory sequences involved in its epxression as well. We also proposed to determine where within such cloned DNA sequences the regulatory sequences lie, the construction of restriction maps on both the functional gene and its regulatory component, and finally the identification and activity of specific DNA sequences which determine the regulated expression of this gene.