REGULATION OF HEPATIC LYMPH LIPOPROTEINS

这项建议的目的是研究载脂蛋白和脂类。 肝淋巴液脂蛋白的组成，并对其进行表征 肝淋巴管插管大鼠作为研究脂蛋白的体内模型 肝脏的分泌物。为了实现这些目标，我们将首先完成 肝淋巴和肝组织载脂蛋白及脂类成分的研究 它的脂蛋白。然后，通过使用隔离的灌流肝脏，但与 肝淋巴管插管后，我们将测量其数量和类型 脂蛋白分泌到淋巴和灌流液(即血管 途径)在已知可修改的正常和实验条件下 肝脏极低密度脂蛋白的产生。我们还将测量等离子体的贡献 利用维生素A标记极低密度脂蛋白对肝淋巴中极低密度脂蛋白的影响 极低密度脂蛋白。为了进一步检验使用肝淋巴管的有效性 大鼠作为研究肝脏脂蛋白分泌的体内模型，我们 将研究肝淋巴极低密度脂蛋白中载脂蛋白和脂质的产量 用高蔗糖饮食、酒精、乙炔对大鼠进行治疗。 雌二醇和4-氨基吡唑并嘧啶。最后，肝淋巴 用去势大鼠研究肠淋巴的作用 分流肝脏的脂蛋白分泌。长期目标 本研究都是利用这一体内模型来研究影响因素 调节肝脏和新陈代谢的脂蛋白的分泌 肝淋巴液中的脂蛋白。

The goals of this proposal are to study the apoprotein and the lipid compositions of hepatic lymph lipoproteins, and also to characterize the hepatic lymph cannulated rat as an in vivo model for studying lipoprotein secretion by the liver. To achieve these goals, we will first finish characterizing the apoprotein and lipid composition of hepatic lymph and its lipoproteins. Then, by using the isolated perfused liver but with the hepatic lymph duct cannulated, we will measure the quantity and the type of lipoproteins secreted into the lymph and the perfusate (i.e., the vascular pathway) under normal and experimental conditions which are known to modify hepatic VLDL production. We will also measure the contribution of plasma VLDL to the VLDL in hepatic lymph through the use of vitamin A labeled VLDL. To further test the validity of using the hepatic lymph cannulated rat as an in vivo model for studying lipoprotein secretion by the liver, we will study the output of apoprotein and lipid in hepatic lymph VLDL when the rats have been treated with a high sucrose diet, alcohol, ethynyl estradiol and 4-aminopyrazolopyrimidine. Lastly, the hepatic lymph cannulated rat will be used to study the effect of intestinal lymph diversion on lipoprotein secretion by the liver. The long-term objectives of this research are the use of this in vivo model to study the factors that regulate the secretion of lipoproteins by the liver and the metabolism of the hepatic lymph lipoproteins.