Cincinnati Mouse Metabolic Phenotyping Center

总体描述（由申请人提供）： 该提案来自辛辛那提的一组知名科学家，是对RFA DK-00-014的回应，该提案旨在继续资助NIH资助的五个小鼠代谢表型中心之一，其使命是为来自AMDCC的不同科学家和研究人员提供与糖尿病及其并发症相关的表型服务。对于辛辛那提小鼠代谢表型中心（MMPC）来说，这是一个令人兴奋的四年半，我们在建立这个项目的过程中克服了许多绊脚石。我们看到，要求提供服务的调查员人数迅速增加，特别是年轻的调查员。此外，应当指出，许多要求辛辛那提MMPC提供服务的调查员来自其他大学。辛辛那提MMPC发展迅速，为每个服务核心增加了新的、利用良好的测试，稍后将在每个核心中详细介绍。例如，我们的脂质和脂蛋白代谢核心（核心C）中增加了粪便脂肪吸收的无创测量。这项测试的纳入对我们来说特别有意义，因为它是从试点和可行性计划赠款的初始资金发展而来的。此外，我们还购买了Luminex 100系统，该系统大大提高了我们仅使用非常小体积的血清（通常为20 ml）即可测定肽和激素循环水平的能力。随着最近招募SilkenObici博士到UC，我们现在已经将葡萄糖钳添加到我们的检测库中。提出了五个核心：1）行政/统计核心将负责处理请求，记录测试结果，进行统计分析，处理预算问题，购买用品和设备，在中心网站上发布有关提供的测试类型和使用的协议的信息; 2）动物护理中心将接收，房屋，饲料，并在表型测试期间对进入的小鼠进行基因分型并使它们保持良好的状态; 3）动物脂质、脂蛋白和葡萄糖代谢Core将研究脂质和葡萄糖代谢并测量已知影响胰岛素分泌的胃肠激素; 4）心血管和肾脏核心将提供心血管和肾脏系统功能的许多评估;食物摄入和行为核心将评估能量摄入的简单和详细参数（食物摄入）、能量消耗（包括使用间接热量测定法）和身体成分和脂肪含量，以及通过CLEVER系统进行的行为测定。我们相信，我们的集体经验和专业知识以及我们在2001年7月1日至2006年6月30日期间运营和运营四个中心之一的经验使我们的建议具有独特性和竞争力，我们对在这一重要倡议上合作的前景感到兴奋。

DESCRIPTION, OVERALL (provided by applicant): This proposal, from a group of established scientists at the University of Cincinnati is in response to RFA DK-00-014 to continue funding for one of five NIH-funded Mouse Metabolic Phenotyping Centers with the mission of providing phenotyping services related to diabetes and its complications to diverse scientists and investigators from the AMDCC. It has been an exciting four and half years for the Cincinnati Mouse Metabolic Phenotyping Center (MMPC), and we have overcome many stumbling blocks while building this program. We have seen rapid growth in the number of investigators requesting service, particularly from young investigators. In addition, it should be noted that many of the investigators requesting services from the Cincinnati MMPC were from other universities. The Cincinnati MMPC evolved rapidly with the addition of new, well utilized tests to each of the service cores which will be detailed later in each Core. For example, the non-invasive measurement of fecal fat absorption was added to our Lipid and Lipoprotein Metabolism Core (Core C). The incorporation of this test was particularly rewarding for us, as it evolved from the initial funding of a Pilot and Feasibility Program grant. Additionally, we have purchased the Luminex 100 System, which has greatly increased our capacity to determine the circulating level of peptides and hormones with the use of only a very small volume of serum (usually 20 ml). With the recent recruitment of Dr. Silvana Obici to UC, we have now added glucose clamps to our repertoire of assays. Five Cores are proposed: 1) Administrative/Statistical Core will be responsible for processing requests, record test results, perform statistical analyses, take care of budgetary issues, purchase of supplies and equipment, post information about the types of tests offered and protocol used on the Center's web site; 2) Animal Care Core will receive, house, feed, and genotype the incoming mice as well as maintain them in excellent condition during the period of phenotypic testing; 3) Animal lipid, lipoprotein, and glucose metabolism Core will study lipid and glucose metabolism and measure gastrointestinal hormones known to influence insulin secretion; 4) Cardiovascular and Renal Core will provide numerous assessments of the functioning of the cardiovascular and renal systems; and 5) Food Intake and Behavioral Core will assess simple and detailed parameters of energy intake (food intake), energy expenditure (including use of indirect calorimetry) and body composition and fat content, and behavioral determinations by the CLEVER system. We believe that our collective experience and expertise as well as our experience running and operating one of the four centers from July, 1 2001 through June 30, 2006 make our proposal unique and competitive, and we are excited at the prospect of collaborating on this important initiative.