Cincinnati Mouse Metabolic Phenotyping Center

总体描述(由申请人提供)： 这项由辛辛那提大学一群知名科学家提出的建议是为了响应RFA DK-00-014，继续资助NIH资助的五个小鼠代谢表型中心之一，其使命是为AMDCC的不同科学家和研究人员提供与糖尿病及其并发症相关的表型服务。对于辛辛那提老鼠代谢表型中心(MMPC)来说，这是令人兴奋的四年半，我们在建立这个项目的过程中克服了许多绊脚石。我们看到要求服务的调查人员数量迅速增加，特别是年轻调查人员。此外，应该指出的是，许多要求辛辛那提MMPC提供服务的调查人员来自其他大学。辛辛那提MMPC随着每个服务核心增加了新的、利用良好的测试而迅速发展，这些测试将在后面的每个核心中详细介绍。例如，我们的脂质和脂蛋白代谢核心(核心C)增加了粪便脂肪吸收的非侵入性测量。纳入这项测试对我们来说特别有意义，因为它是从试点和可行性计划拨款的初始资金演变而来的。此外，我们购买了Luminex 100系统，这极大地提高了我们仅使用非常少量的血清(通常为20毫升)就可以测定多肽和激素循环水平的能力。随着西尔瓦纳·奥比奇博士最近加入加州大学，我们现在将葡萄糖钳添加到我们的检测体系中。提议了五个核心：1)行政/统计核心将负责处理请求，记录测试结果，进行统计分析，处理预算问题，购买用品和设备，在中心的网站上张贴有关提供的测试类型和使用的方案的信息；2)动物护理核心将接收、安置、喂养进入中心的小鼠，并在表型测试期间保持它们的良好状态；3)动物脂肪、脂蛋白和葡萄糖代谢核心将研究脂肪和葡萄糖代谢，并测量已知影响胰岛素分泌的胃肠激素；4)心血管和肾脏核心将提供对心血管和肾脏系统功能的大量评估；以及5)食物摄入量和行为核心将评估简单和详细的参数，如能量摄入量(食物摄入量)、能量消耗(包括使用间接量热法)、身体成分和脂肪含量，以及由智能系统进行的行为决定。我们相信，我们的集体经验和专业知识，以及我们在2001年7月1日至2006年6月30日期间运营和运营四个中心之一的经验，使我们的提案具有独特性和竞争力，我们对在这一重要倡议上进行合作的前景感到兴奋。

DESCRIPTION, OVERALL (provided by applicant): This proposal, from a group of established scientists at the University of Cincinnati is in response to RFA DK-00-014 to continue funding for one of five NIH-funded Mouse Metabolic Phenotyping Centers with the mission of providing phenotyping services related to diabetes and its complications to diverse scientists and investigators from the AMDCC. It has been an exciting four and half years for the Cincinnati Mouse Metabolic Phenotyping Center (MMPC), and we have overcome many stumbling blocks while building this program. We have seen rapid growth in the number of investigators requesting service, particularly from young investigators. In addition, it should be noted that many of the investigators requesting services from the Cincinnati MMPC were from other universities. The Cincinnati MMPC evolved rapidly with the addition of new, well utilized tests to each of the service cores which will be detailed later in each Core. For example, the non-invasive measurement of fecal fat absorption was added to our Lipid and Lipoprotein Metabolism Core (Core C). The incorporation of this test was particularly rewarding for us, as it evolved from the initial funding of a Pilot and Feasibility Program grant. Additionally, we have purchased the Luminex 100 System, which has greatly increased our capacity to determine the circulating level of peptides and hormones with the use of only a very small volume of serum (usually 20 ml). With the recent recruitment of Dr. Silvana Obici to UC, we have now added glucose clamps to our repertoire of assays. Five Cores are proposed: 1) Administrative/Statistical Core will be responsible for processing requests, record test results, perform statistical analyses, take care of budgetary issues, purchase of supplies and equipment, post information about the types of tests offered and protocol used on the Center's web site; 2) Animal Care Core will receive, house, feed, and genotype the incoming mice as well as maintain them in excellent condition during the period of phenotypic testing; 3) Animal lipid, lipoprotein, and glucose metabolism Core will study lipid and glucose metabolism and measure gastrointestinal hormones known to influence insulin secretion; 4) Cardiovascular and Renal Core will provide numerous assessments of the functioning of the cardiovascular and renal systems; and 5) Food Intake and Behavioral Core will assess simple and detailed parameters of energy intake (food intake), energy expenditure (including use of indirect calorimetry) and body composition and fat content, and behavioral determinations by the CLEVER system. We believe that our collective experience and expertise as well as our experience running and operating one of the four centers from July, 1 2001 through June 30, 2006 make our proposal unique and competitive, and we are excited at the prospect of collaborating on this important initiative.