Cincinnati Mouse Metabolic Phenotyping Center

辛辛那提小鼠代谢表型中心

• 批准号: 7930188
• 负责人: PATRICK TSO
• 金额: $ 24.4万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7930188
• 关键词: Animals; Behavioral; Biological Assay; Body Composition; Cardiovascular Physiology; Cardiovascular system; Caring; Diabetes Mellitus; Eating; Energy Intake; Energy Metabolism; Equipment and Supplies; Euglycemic Clamping; Fatty acid glycerol esters; Funding; Gastrointestinal Hormones; Genotype; Glucose Clamp; Grant; Housing; Indirect Calorimetry; Kidney; Lipids; Lipoproteins; Measurement; Measures; Metabolic; Metabolism; Mission; Mus; Phenotype; Process; Protocols documentation; Research Personnel; Rewards; Running; Scientist; Serum; Services; System; Test Result; Testing; Universities; absorption; animal care; experience; feeding; glucose metabolism; insulin secretion; peptide hormone; programs; rapid growth; response; web site

DESCRIPTION, OVERALL (provided by applicant): This proposal, from a group of established scientists at the University of Cincinnati is in response to RFA DK-00-014 to continue funding for one of five NIH-funded Mouse Metabolic Phenotyping Centers with the mission of providing phenotyping services related to diabetes and its complications to diverse scientists and investigators from the AMDCC. It has been an exciting four and half years for the Cincinnati Mouse Metabolic Phenotyping Center (MMPC), and we have overcome many stumbling blocks while building this program. We have seen rapid growth in the number of investigators requesting service, particularly from young investigators. In addition, it should be noted that many of the investigators requesting services from the Cincinnati MMPC were from other universities. The Cincinnati MMPC evolved rapidly with the addition of new, well utilized tests to each of the service cores which will be detailed later in each Core. For example, the non-invasive measurement of fecal fat absorption was added to our Lipid and Lipoprotein Metabolism Core (Core C). The incorporation of this test was particularly rewarding for us, as it evolved from the initial funding of a Pilot and Feasibility Program grant. Additionally, we have purchased the Luminex 100 System, which has greatly increased our capacity to determine the circulating level of peptides and hormones with the use of only a very small volume of serum (usually 20 ml). With the recent recruitment of Dr. Silvana Obici to UC, we have now added glucose clamps to our repertoire of assays. Five Cores are proposed: 1) Administrative/Statistical Core will be responsible for processing requests, record test results, perform statistical analyses, take care of budgetary issues, purchase of supplies and equipment, post information about the types of tests offered and protocol used on the Center's web site; 2) Animal Care Core will receive, house, feed, and genotype the incoming mice as well as maintain them in excellent condition during the period of phenotypic testing; 3) Animal lipid, lipoprotein, and glucose metabolism Core will study lipid and glucose metabolism and measure gastrointestinal hormones known to influence insulin secretion; 4) Cardiovascular and Renal Core will provide numerous assessments of the functioning of the cardiovascular and renal systems; and 5) Food Intake and Behavioral Core will assess simple and detailed parameters of energy intake (food intake), energy expenditure (including use of indirect calorimetry) and body composition and fat content, and behavioral determinations by the CLEVER system. We believe that our collective experience and expertise as well as our experience running and operating one of the four centers from July, 1 2001 through June 30, 2006 make our proposal unique and competitive, and we are excited at the prospect of collaborating on this important initiative.

描述，总体（由申请人提供）： 这项提案是从辛辛那提大学的一组知名科学家开始的，这是对RFA DK-00-014的回应，继续为五个NIH资助的NIH资助的小鼠代谢表型中心之一提供资金，其使命是提供与糖尿病及其复杂性的表型服务，以使其与AMDCC的糖尿病及其复杂性有关。对于辛辛那提小鼠代谢表型中心（MMPC）来说，这已经是令人兴奋的四年半，并且在构建该程序时，我们已经克服了许多绊脚石。我们已经看到，要求服务的调查人员数量迅速增长，尤其是来自年轻的调查人员。此外，应该指出的是，许多要求辛辛那提MMPC服务的调查人员来自其他大学。辛辛那提MMPC随着在每个服务核心中添加了新的，良好的测试而迅速发展，这些测试将在后面在每个核心中进行详细介绍。例如，将粪便吸收的非侵入性测量添加到我们的脂质和脂蛋白代谢核心（核心C）中。这项测试的合并对我们特别有意义，因为它是从试点和可行性计划赠款的最初资助而发展而来的。此外，我们购买了Luminex 100系统，该系统仅使用很少的血清（通常为20 mL）来大大提高了确定肽和激素循环水平的能力。随着最近将Silvana Obici博士招募到UC，我们现在为我们的测定曲目添加了葡萄糖夹。提出了五个核心：1）行政/统计核心将负责处理请求，记录测试结果，执行统计分析，照顾预算问题，购买用品和设备，发布有关所提供的测试类型以及该中心网站上使用的协议的信息； 2）动物护理核心将接收，房屋，饲料和基因型，在表型测试期间将它们保持在良好状态； 3）动物脂质，脂蛋白和葡萄糖代谢核心将研究脂质和葡萄糖代谢，并测量已知会影响胰岛素分泌的胃肠道激素； 4）心血管和肾脏核心将对心血管和肾脏系统的功能进行大量评估； 5）食物摄入量和行为核心将评估能量摄入量（食物摄入量），能量消耗（包括使用间接量热法），身体成分和脂肪含量以及智能系统的行为确定的简单而详细的参数。我们认为，从2001年7月1日至2006年6月30日，我们的集体经验和专业知识以及运行和运营四个中心之一的经验使我们的提议变得独特而竞争，我们为合作制定这一重要倡议而感到兴奋。