CLONING OF THE HMG CO-A SYNTHASE GENE

这个项目的长期目标是扩大我们对 胆固醇生物合成酶的遗传调节。 此类研究 它们的科学意义来自于一些不寻常的机会 在该系统中研究基因表达的转录调控， 利用从这些研究中获得的见解， 了解发病机制和最终的化疗 动脉粥样硬化性心血管疾病 具体来说，这个项目 描述了针对克隆人类酶基因的研究 3-羟基-3-甲基戊二酰辅酶A酶的DNA技术 介导的基因转移 所提议的方法提供了一种手段， 仅用于克隆基因的信息元件，但用于克隆 调控序列参与其表达。 我们亦建议 为了确定在这些克隆的DNA序列中， 序列的谎言，构建限制性地图上的功能 基因及其调控成分，最后鉴定和 决定调节表达的特定DNA序列的活性 这个基因。

The long range goal of this project is to enlarge our understanding of the genetic regulation of enzymes in cholesterol biosysnthesis. Such studies derive their scientific significance both from some unusual opportunities in this system to study transcriptional control of gene expession and the utilization of insights derived from these studies to increase our understanding of the pathogenesis and eventual chemotherapy of atherosclerotic cardiovascular disease. Specifically, this project describes studies directed at cloning the human gene for the enzyme 3-hydroxy-3-methyglutaryl coenzyme A synthases by the technique of DNA mediated gene transfer. The methodology proposed provides a vehicle not only for cloning informational elements of the gene, but for cloning regulatory sequences involved in its epxression as well. We also proposed to determine where within such cloned DNA sequences the regulatory sequences lie, the construction of restriction maps on both the functional gene and its regulatory component, and finally the identification and activity of specific DNA sequences which determine the regulated expression of this gene.