MECHANISMS OF NEUTROPHIL ADHERENCE

中性粒细胞与内皮细胞的黏附是必要的步骤 中性粒细胞向炎症部位迁移。与之形成鲜明对比的是 增强自然宿主对感染的抵抗力 血液透析患者中性粒细胞的黏附和聚集 白细胞减少被认为是导致急性肺病发展的原因。 由于增加了封存而造成的损害，以及随后对 内皮细胞。涉及中性粒细胞的类似损伤机制也是如此 与成人呼吸窘迫综合征(ARDS)有关，免疫 在复杂疾病和急性心肌梗死中。该计划的目标是 拟议的研究是确定人类中性粒细胞 附着在血管表面。拟议的研究将包括 细胞表面相关蛋白的纯化与鉴定 中性粒细胞在包括培养的人内皮细胞表面的黏附 细胞。自然产生的非补体衍生血清因子的作用 内皮细胞因子在调节促分泌剂诱导的中性粒细胞中的作用 遵守也将被确定。此外，实验将是 以确定中性粒细胞与表面的粘连是否会导致 在细胞骨架结构的重排中。这些研究应该会产生 中性粒细胞与内皮细胞黏附机制的新进展 细胞被认为在许多疾病的发病机制中起重要作用 中性粒细胞介导的血管疾病，如ARDS和 中性粒细胞激活可能起致病作用。他们还可以提供 寻找更合理的治疗方式的线索。

Adherence of neutrophils to endothelial cells represents a necessary step in the emigration of neutrophils to inflammatory sites. In contrast to augmentation of natural host defense to infection, the intensified adhesiveness and aggregation of neutrophils noted with hemodialysis leukopenia has been suggested to result in the development of acute lung injury by virtue of increased sequestration and subsequent damage to endothelium. Similar mechanisms of injury involving neutrophils have been implicated in the Adult Respiratory Distress Syndrome (ARDS), immune complex diseases and in acute myocardial infarction. The objective of the proposed research is to determine mechanisms by which human neutrophils adhere to vascular surfaces. The proposed studies will involve purification and characterization of cell-surface proteins involved in adherence of neutrophils to surfaces including cultured human endothelial cells. The role of naturally occurring noncomplement derived serum factors and endothelial cell factors in modulating secretagogue induced neutrophil adherence will also be determined. Furthermore, experiments will be performed to determine whether adherence of neutrophils to surfaces results in rearrangement of cytoskeletal structures. These studies should yield new information regarding mechanisms of neutrophil adherence to endothelial cells thought to be important in the pathogenesis of many neutrophil-mediated vascular diseases, such as ARDS and diseases in which neutrophil activated may play a pathogenetic role. They may also provide clues to more rational forms of therapy.