IMMUNE FUNCTIONS OF ACCESSORY CELLS IN THE LUNG

我们的研究目的是分析 辅助细胞，并将其与免疫反应联系起来 由吸入的病原体和外来抗原引发 附件 在大多数免疫应答的诱导过程中，细胞必须表达MHC II类抗原（Ia）在它们的表面上并且具有内吞的能力， 处理并将抗原呈递给T细胞。 具体的目的是鉴定刘易斯大鼠肺中的那些细胞， 具有呈递抗原和分析缺陷的能力， 使肺泡巨噬细胞（AM）不能有效地呈递抗原 到T细胞。 这将通过使用天然鸡蛋溶菌酶来完成 (HEL)和含有主要T细胞表位的肽片段。 的 将通过免疫T细胞来测量呈递抗原的能力 增殖测定。 双标记免疫凝胶技术将使用高 亲和性兔抗FITC抗体和单克隆鼠抗大鼠Ia 抗体来识别FITC-HEL通过的细胞区室 在细胞表面呈递之前通过，并确定它是否 在细胞内的任何时候都与Ia相关联。 的性质 将检测AM表面上T细胞的推定结合活性。 细胞分离技术将用于分离间质巨噬细胞， 树突状细胞和肺泡II型肺细胞，以测试它们的能力 或者表达Ia组成型，或者在用 富含干扰素的淋巴因子。 他们将被检查他们的能力， 将HEL抗原呈递给HEL免疫T细胞并表达白细胞介素I 活动 最后，这些细胞与免疫T细胞的相互作用将 在HEL-immunized刘易斯大鼠中进行检测， 施用与15 nm金共价连接的可溶性或不溶性HEL 粒子 这些研究的结果应有助于更好地了解 AM经历的适应性变化导致他们未能 有效地呈递抗原。 他们还将识别出这些细胞中的 肺，其充当感受态抗原呈递细胞。

The objective of our research is to analyze the immune function of accessory cells in the lung and to relate these to immune responses triggered as a result of inhaled pathogens and foreign antigens. Accessory cells, during the induction of most immune responses, must express MHC class II antigens (Ia) on their surface and have the ability to endocytose, process and present antigen to T-cells. The specific aims are to identify those cells in the Lewis rat lung which have the ability to present antigen ad to analyze the defect(s) which render the alveolar macrophage (AM) unable to effectively present antigen to T-cells. This will be accomplished by using native hen egg lysozyme (HEL) and peptide fragments containing the major T-cell epitope. The ability to present antigen will be measured by means of an immune T-cell proliferation assay. Double labelling immunogols techniques will use high affinity rabbit anti-FITC antibodies and monoclonal mouse anti-rat Ia antibodies to identify the cellular compartments through which FITC-HEL passes before presentation on the cell surface and to establish whether it becomes associated with Ia at any time within the cell. The nature of a putative binding activity for T cells on the AM surface will be examined. Cell isolation techniques will be used to isolate interstitial macrophages, dendritic cells, and alveolar type II pneumocytes to test for their ability to either express Ia constituitively or following stimulation with interferon-enriched lymphokine. They will be examined for their ability to present HEL antigen to HEL immune T-cells and to express interleukin I activity. Finally the interaction of these cells with immune T-cells will be examined in HEL-immunized Lewis rats challenged by the intratrached administration of soluble or insoluble HEL covalently linked to 15 nm gold particles. Results from these studies should lead to a better understanding of the adaptations AM have undergone that have resulted in their failure to present antigen effectively. They will also identify those cells in the lung which serve as competent antigen presenting cells.