MECHANISMS OF NEUTROPHIL ADHERENCE

中性粒细胞粘附到内皮细胞是一个必要的步骤 中性粒细胞迁移到炎症部位。 相比 增强自然宿主对感染的防御， 血液透析中观察到中性粒细胞的粘附和聚集 白细胞减少症被认为会导致急性肺 由于隔离增加而造成的伤害以及随后对 内皮细胞 涉及中性粒细胞的损伤的类似机制已经被发现。 成人呼吸窘迫综合征（ARDS），免疫 复杂疾病和急性心肌梗死。 的目的 拟议的研究是确定人类中性粒细胞 粘附在血管表面。 拟议的研究将涉及 参与的细胞表面蛋白的纯化和表征 嗜中性粒细胞对包括培养的人内皮细胞在内的表面的粘附 细胞 天然存在的非补体衍生血清因子的作用 内皮细胞因子在调节促分泌素诱导的中性粒细胞中的作用 还将确定遵守情况。 此外，实验将 用于确定中性粒细胞粘附于表面是否导致 在细胞骨架结构的重排中。 这些研究应该会产生 关于中性粒细胞粘附内皮细胞机制的新信息 细胞被认为是重要的发病机制，许多 嗜中性粒细胞介导的血管疾病，如ARDS和其中 中性粒细胞活化可能起致病作用。 其还可提供 更理性的治疗方式的线索

Adherence of neutrophils to endothelial cells represents a necessary step in the emigration of neutrophils to inflammatory sites. In contrast to augmentation of natural host defense to infection, the intensified adhesiveness and aggregation of neutrophils noted with hemodialysis leukopenia has been suggested to result in the development of acute lung injury by virtue of increased sequestration and subsequent damage to endothelium. Similar mechanisms of injury involving neutrophils have been implicated in the Adult Respiratory Distress Syndrome (ARDS), immune complex diseases and in acute myocardial infarction. The objective of the proposed research is to determine mechanisms by which human neutrophils adhere to vascular surfaces. The proposed studies will involve purification and characterization of cell-surface proteins involved in adherence of neutrophils to surfaces including cultured human endothelial cells. The role of naturally occurring noncomplement derived serum factors and endothelial cell factors in modulating secretagogue induced neutrophil adherence will also be determined. Furthermore, experiments will be performed to determine whether adherence of neutrophils to surfaces results in rearrangement of cytoskeletal structures. These studies should yield new information regarding mechanisms of neutrophil adherence to endothelial cells thought to be important in the pathogenesis of many neutrophil-mediated vascular diseases, such as ARDS and diseases in which neutrophil activated may play a pathogenetic role. They may also provide clues to more rational forms of therapy.