FUNCTIONAL ANALYSIS OF VASCULAR ANGIOTENSIN II

本次调查的目的是确定 血管生成的血管紧张素II在血管紧张素转换酶中的作用 全身动脉压(BP)、基础血管张力和 肾上腺素能反应性。肾素释放的刺激物 所有来自完整血管床的都将被研究。因为. 其高反应性肾素-血管紧张素系统及稳定性 在取回血液样本期间的心血管系统 所需的化验，兔子将被利用。股动脉 在戊巴比妥中将监测灌流压力和血压 麻醉的兔子。股静脉分流术将允许 卡托普利后肢给药不再循环 从而允许选择性血管紧张素转换酶 抑制力。肾切除和假手术兔将被 用来比较所有在 后肢血管构筑的基本参数。为了消除 肾切除术后并发症对电解质平衡和血浆的影响 尿素和进一步诱导血管系统AII的影响， 动物在每次实验前都要进行腹膜透析。 术后1、2、4天。关于血管的干预措施有待研究 肾素和AII释放将是β1和β2-肾上腺素受体 刺激，交感神经的直接电刺激， 出血，传入神经刺激，钙和 钠和戈德布拉特高血压。随之而来的变化 血压、股血管阻力和股血管反应 肾上腺皮质神经刺激和去甲肾上腺素 下定决心。卡托普利和沙拉辛将作为一种手段 区分血管肾素的参与- 这些间歇期的血管紧张素系统。因为 血管紧张素Ⅱ在血压和血压调节中的作用 疾病状态下的血管张力，如高血压和 充血性心力衰竭，可能在基础状态下， 了解这个系统是很重要的。

The purpose of this investigation is to determine the functional role of angiotensin II (AII) generated in the vasculature on systemic arterial pressure (BP), basal vascular tone and adrenergic reactivity. Stimuli responsible for the release of renin and AII from the intact vascular bed will be studied. Because of its highly reactive renin-angiotensin system and stable cardiovascular system during withdrawal of blood samples for the required assays, the rabbit will be utilized. Femoral artery perfusion pressure and BP will be monitored in pentobarbital anesthetized rabbits. A femoral venous shunt will allow for administration of captopril to the hindlimb without recirculating and thus allow for selective angiotensin converting enzyme inhibition. Nephrectomized and sham operated rabbits will be employed to compare the relative importance of AII formed in the hindlimb vasculature on basal parameters. In order to eliminate complications of nephrectomy on electrolyte balance and plasma urea and to further induce the influence of vasculature AII, the animals will undergo peritoneal dialysis before each experiment at 1, 2 and 4 days post op. Interventions to be studied on vascular renin and AII release will be beta 1 and beta 2-adrenoceptor stimulation, direct electrical stimulation of sympathetic nerves, hemorrhage, afferent nerve stimulation, changes in calcium and sodium and Goldblatt hypertension. The accompanying changes in BP, femoral vascular resistence and femoral vascular responses to adrengeric nerve stimulation and norepinehrine will be determined. Captopril and saralasin will be employed as a means of distinguishing the involvement of the vascular renin- angiotensin system during these intervetnions. Because of the postulated role of vascular AII in the regulation of BP and vascular tone in disease states such as hypertension and congestive heart failure and possibly under basal conditions, knowledge about this system is important.