ELECTROPHYSIOLOGIC STUDIES OF PSYCHOMOTOR PROCESSES

该提案的主要目标是定义解剖学 结构和神经活动的基础上记录的表面 事件相关电位（ERP）与感觉、运动 和认知过程。 这些研究使用未麻醉的， 行为的旧世界猴子（M. fascicularis），作为最接近的 人类ERP的可行模型。 在曲面映射后， 具体的ERP研究中，深度记录， 定位颅内发生器，并通过三个定义- 三维脑内标测，野间关系 电位记录在附近的活动 结构和表面记录的电位，通常 表示来自多个结构的复合活动。 的 每个活性结构内的神经过程进一步 通过同时记录场电位和MUA描绘 利用可移动的多触点， 我们自己设计的电极。 局部外向和内向神经元 跨膜电流的估计，从外地 潜在的数据，利用电流源密度分析。 活动构造的识别和区域划分 增强的神经代谢 通过2-脱氧葡萄糖实现电生理分析 在处死每只动物之前进行放射自显影。 着力 用于识别参与产生 具体的ERP功能。 最初的重点是GABA在以下方面的作用： 皮质内抑制回路，采用局部 皮质内注射GABA拮抗剂荷包牡丹碱。 这 免疫细胞化学检查将有助于奋进 大脑中特定神经递质的分布 电生理研究下的结构。 研究将集中在 纹状体诱发反应刺激相关层模式 和纹外视皮层，大脑活动与 训练的自发性手部动作和相关的电位 视觉和听觉辨别任务的表现。

The principal goal of this proposal is to define the anatomical structures and neural activity that underlie the surface recorded Event Related Potentials (ERP) associated with sensory, motor and cognitive processes. These studies utilize unanesthetized, behaving old world monkeys (M. fascicularis), as the closest feasible model for human ERP. After surface mapping of the specific ERP under study, depth recordings are employed to localize the intracranial generators and to define through three- dimensional intracerebral mapping, the relationship between field potentials recorded in the immediate vicinity of the active structures and the surface recorded potentials, which usually represent composite activity from more than one structure. The neural processes within each active structure are further delineated by concurrent recording of field potentials and MUA from closely spaced electrodes, utilizing a moveable multicontact electrode of our own design. Local outward and inward neural transmembrane current flows are estimated from the field potential data, utilizing current source density analysis. Identification of active structures and delineation of regions of enhanced neural metabolism within the structures under electrophysiological analysis is achieved by 2-Deoxyglucose autoradiography prior to sacrifice of each animal. Efforts will be made to identify the neurotransmitters involved in generation of specific ERP features. The initial focus is on the role of GABA in intracortical inhibitory circuitry, employing localized intracortical injections of the GABA antagonist bicuculline. This endeavor will be facilitated by immunocytochemical examination of the distribution of specific neurotransmitters within the brain structures under elecrophysiologic study. Studies will focus on stimulus related laminar patterns of evoked response in striate and extrastriate visual cortex, cerebral activity associated with trained self-initiated hand movements and the potentials related to the performance of visual and auditory discrimination tasks.