BLOOD CELL MECHANICS, SURFACE AFFINITY AND ADHESION

血细胞的变形性是重要的决定因素 微循环中的血流分布，并调节细胞 聚集、凝聚和吞噬相互作用。这个 这项研究的目的是量化与时间相关的 白血球的机械性能，能量学 血细胞变形与细胞内化学吸引 粘合，以及粘接触点分离的机理。 细胞聚集的自由能势量化了 细胞表面识别的初始阶段是一个重要的 在去除异常细胞和物质中的因素 循环、细胞被病原体感染、停滞和 循环中的白色细胞和肿瘤细胞的边际化。优势所在 粘附力(由分离所需的力表示 接触)决定了细胞聚集体的活性 暴露在循环中的剪切力的干扰下。 具体目标旨在解决以下一般问题 问：被动语态的共性和多样性是什么？ 白血球的流变性？在那里吗 血液吞噬细胞之间的区别特征？什么是 表示主动机械应力的固有属性 由吞噬细胞产生，这些特性如何相关 细胞内的化学反应？行动的亲和力是什么？ 在远距离通过特定的凝集促进粘连 有什么反应吗？每座交叉桥的“断裂”能量是多少 稳定粘性接触，以对抗环境压力？ 撞击力如何在凝集反应中发挥作用？ 独特的微操作技术被用于执行 单细胞变形性和细胞间的细观力学测试 粘附力。观察结果用视频显微镜记录下来 系统。配体-受体的表面结合和分布 用视频增强型激光测量复合体 荧光测量系统，与显微镜系统耦合。

Deformability properties of blood cells are essential determinants of flow distribution in the microcirculation and also modulate cell aggregation, agglutionation, and phagocytic interactions. The objectives of this research are to quantitate the time-dependent mechanical properties of white blood cells, the energetics of blood cell deformation and chemical attraction in cell-cell adhesion, and the mechanics of separation of adhesive contacts. The free energy potential for cell aggregation quantitates the initial stage of cell-surface "recognition" which is an important factor in the removal of aberrant cells and material from the circulation, infection of cells by pathogens, arrest and margination of circulating white and tumour cells. The strength of adhesion (represented by the forces required to separate contacts) determines the viability of cell aggregates when exposed to disruption by shear forces in the circulation. The specific aims are designed to address the following general questions: what is the commonality and diversity of passive rheological properties amongst white blood cells? Are there distinguishing features between blood phagocytes? What are the intrinsic properties that represent active mechanical stresses generated by phagocytes and how can these properties be related to chemical reactions inside cells? What is the affinity that acts at long range to promote adhesion via specific agglutination reactions? What is the "fracture" energy per crossbridge that stabilizes adhesive contacts in oppostion to environmental forces? How do impingement forces play a role in agglutination reactions? Unique micromanipulation techniques are used to perform micromechanical tests of single cell deformability and cell-cell adhesion. The observations are recorded with a video microscope system. Surface binding and distribution of ligand-receptor complexes are measured with a video intensified, laser fluorometry system, coupled to the microscope system.