DEVELOPMENT OF SYMPATHETIC JUNCTIONS IN CULTURE

最近对交感神经元的研究表明，它们的递质状态 比目前教科书上的观点更复杂；有证据表明 新的递质(如嘌呤、多肽)、多个递质状态、 可塑性和分级表达。长期目标是 用本研究中设计的“微培养”方法研究这些复杂性 实验室允许对每一种新的 与适当的靶细胞共培养的单个神经元的复杂性。 从围产期或成年大鼠神经节分离出的神经元将会生长， 一次一个或几个，在目标细胞的微小孤岛上(例如其他 神经元、心肌或平滑肌细胞)；递质状态由 刺激神经元并从神经元和其上电记录 靶细胞，并使用适当的药物。精细的结构或 然后，免疫细胞化学可以直接与血管内皮细胞的功能相关 这个神经元。具体目标： 1.关于肾上腺素能向胆碱能的转变，研究 I)某些肾上腺素能特性的保留。二)过渡是否可以 颠倒了？三)成年神经元是从特定目标反向标记的吗 递质状态下的组织同质性及其转变(S)？四)在一个 多功能神经元，是单个静脉曲张的功能倍数吗？一个 单泡的？ 2.关于嘌呤能功能(S)的药理研究 是否存在单独的P1和P2受体？摄取 机械装置？二)研究藏品的储存机制(S) 嘌呤(S)。与健康相关。发射机的这些新的复杂性 状态在神经生物学中是普遍感兴趣的。正确理解 对内脏的神经元控制取决于对这些的理解 很复杂。

Recent work on sympathetic neurons indicates that their transmitter status is more complicated than the current textbook views; there is evidence for new transmitters, (e.g. purines, peptides), multiple-transmitter status, plasticity and graded expression. The long term objective is to investigate these complexities with a "microculture" method devised in this laboratory that permits an unusually direct study of each of these new complexities in single neurons co-cultured with appropriate target cells. Neurons dissociated from perinatal or adult ganglia of rats will be grown, one or a few at a time, on tiny islands of target cells (e.g. other neurons, cardiac or smooth myocytes); transmitter status is determined by stimulating the neuron and recording electrically from the neuron and its target cells, with use of appropriate drugs. The fine structure or immunocytochemistry can be then correlated directly with the function of this neuron. Specific aims: 1. With regard to the adrenergic-to-cholinergic transition, to investigate i) Retention of certain adrenergic properties. ii) Can the transition be reversed? iii) Are adult neurons back-labelled from a particular target tissue homogeneous in transmitter status and its transition(s)? iv) In a multiple-function neuron, is a single varicosity multiple in function? A single vesicle? 2. With regard to purinergic function(s) to invetigate i) The pharmacology of purinergic function; are there separate P1 and P2 -receptors? Uptake mechanism? ii) Investigate the storage mechanism(s) for secreted purine(s). Health relatedness. These new complexities of transmitter status are of general interest in neurobiology. A proper understanding of neuronal control of internal organs depends on an understanding of these complexities.