MOLECULAR BASIS OF CELL RECOGNITION IN DEVELOPMENT

这项工作的主要长期目标是了解 控制重要神经元特性的表达 哺乳动物中枢神经系统的发育。我们计划使用 识别神经元亚群的单抗 确定细胞特异性抗原首次表达的时间。特别是， 我们希望找出在分裂过程中是否存在细胞特异性抗原的表达 干细胞是成熟神经元的祖细胞，或者如果最终 有丝分裂必须在细胞特异性抗原表达开始之前发生。我们 我还想知道是否形成了适当的联系 启动或维持细胞表达所必需的 特定的抗原。 提案的一部分是使用两个新的 发现了单抗。RB-8对大鼠嗅觉细胞亚群进行染色 他们的轴突。我们将研究Rb-8染色在卵巢癌组织中的分布。 胚胎和成年嗅球。在成人中，我们将绘制 Rb-8阳性纤维分布于整个球部。 我们将在几只成年大鼠身上这样做，看看这种模式是否不变 或者它是否因动物不同而不同。Rb-8染色的研究进展 将在胚胎和幼年动物身上仔细观察。最后， 再生嗅觉投射中的Rb-8染色模式将是 下定决心。之所以选择RB-6，是因为在视网膜中，它只对 视网膜神经节细胞。在RB-6的情况下，我们将观察到 出生前、出生期间和出生后视网膜中抗原阳性细胞的变化 神经节细胞。Rb-6抗原的体外表达也将是 研究以确定靶细胞的存在是否会影响抗原 表情。 识别细胞表面抗原的单抗 神经元在未来这一领域的研究中将被证明是重要的。我们的一部分 将致力于选择新的单抗和 这些属性。我们将同时使用神经肿瘤和来自 开发大脑来免疫小鼠并产生更多的杂交瘤。全 现在将使用大鼠的细胞和膜作为大鼠进行工作 似乎是目前对中枢神经系统生物学研究最深入的脊椎动物 是令人担忧的。

The major long range goal of this work is to understand the mechanisms that control the expression of important neuronal properties during the development of the mammalian central nervous system. We plan to use monoclonal antibodies (MAbs) which recognize subsets of neurons to determine when cell specific antigens are first expressed. In particular, we wish to find out if cell specific antigen expression occurs in dividing stem cells that are the progenitors of the mature neuron or if the final mitosis must occur before cell specific antigen expression begins. We would also like to find out if the formation of appropriate connections is necessary in order to either initiate or maintain expression of cell specific antigens. One part of the proposal is to investigate these questions using two newly discovered MAbs. RB-8 stains a subset of rat olfactory sensory cells and their axons. We will study the distribution of RB-8 staining in the embryonic and adult olfactory bulb. In the adult we will map the distribution of RB-8 positive fibers over the entire extent of the bulb. We will do this in several adult rats to see if this pattern is invariant or if it varies from animal to animal. The development of RB-8 staining will be carefully followed in embryonic and young animals. Finally, the pattern of RB-8 staining in regenerating olfactory projections will be determined. RB-6 was seleted because, in the retina, it stains only the retinal ganglion cells. In the case of RB-6 we will observe the appearance of antigen positive cells in retinas before, during and after the birth of ganglion cells. The expression of RB-6 antigen in vitro will also be studied to determine if the presence of target cells influences antigen expression. Monoclonal antibodies which recognize cell surface antigens on subsets of neurons will prove important in future research in this area. Part of our efforts will be directed towards selecting new monoclonal antibodies with these attributes. We will use both neural tumors and membranes from the developing brain to immunize mice and produce additional hybridomas. All work will be carried out using rat cells and membranes as the rat now appears to be the most intensively studied vertebrate as far as CNS biology is concerned.