SALIVA NERVE GROWTH FACTOR, THE NATURALLY OCCURRING FORM

一些证据表明，神经生长因子的分离 显示出多种生物学功能， 几个与健康有关的功能。 这些活动包括：1）激活 纤溶酶原; 2）促进伤口愈合速度; 3）神经突 生长刺激活性; 4）激活经典的 补体;和5）对人白细胞的趋化活性。 本文所载的研究建议旨在提高我们的 了解NGF的这些潜在生物学作用。 实验旨在加深我们对化学物质的理解 神经生长因子发生自催化激活的机制及其控制 锌离子。 研究还提出了旨在了解神经生长因子的分离 来自人血清的Cohn组分III和来自牛精浆。 最后，实验室的一个主要目标是探索和了解我们的 最近发现神经生长因子对 多形核白细胞和单核细胞。 这些研究， 我们发现，NGF可以直接参与激活 补体系统，可能有助于我们理解的重大 NGF在伤口愈合过程中的作用

Several lines of evidence now indicate that nerve growth factor isolated from mouse salivary glands displays multiple biologic functions which span several health-related functions. These activities include: 1) activation of plasminogen; 2) promotion of the rate of wound healing; 3) neurite outgrowth stimulatory activity; 4) activation of the classical pathway of complement; and 5) chemotactic activity toward human leukocytes. The research proposal contained herein is designed to enhance our understanding of each of these potential biologic roles of NGF. Experiments are designed to further our understanding of the chemical mechanism whereby NGF undergoes autocatalytic activation - and its control by zinc ion. Studies are also presented which aim to understand the isolation of NGF from Cohn Fraction III of human serum and from bovine seminal plasma. Finally, a major goal of the laboratory is to explore and to understand our recent finding that nerve growth factor is strongly chemotactic for polymorphonuclear leukocytes and for monocytes. These studies, together with our finding that NGF can participate directly in activation of the complement system, may contribute significantly to our understanding of the role of NGF in the wound healing process.