BRAIN TRANSPLANT TREATMENT FOR PARKINSONIANS

描述（研究者摘要）：本更新期的目标 将研究环孢素A的免疫抑制价值和基础 成纤维细胞生长因子在改善细胞存活和行为 移植胎儿中脑多巴胺细胞后的表现， 帕金森病的猴子模型 这些研究是一个直接的延伸 我们实验室将胎儿细胞移植到 MPTP损伤猴。 大多数从事猴子移植工作的小组都有 发现胎儿多巴胺细胞在发育早期（6到9周）， 妊娠期）可以存活在成年人的大脑中，并产生一些行为效应。 尽管如此，恢复并不完全，细胞的存活率 猴子之间的差异很大。 免疫抑制剂的价值 用于促进细胞存活和增强行为改善的疗法， 在猴子身上从未被探索过。 碱性成纤维细胞生长因子已被证明在 改善大鼠胚胎中脑多巴胺细胞组织中的存活 培养和大鼠中以改善胎儿神经元移植物。 为这些 实验中，我们将从全身性MPTP损伤的猴子切换到 用神经毒素MPTP单侧损伤的猴子 注入颈动脉 MPTP模型发生这种变化的原因 是因为很难得到一个可复制的稳定的帕金森 在全身性病变动物中中度严重程度的综合征。 移植 成功将通过在一个新的运动中学习性能的变化来评估 我们设计的任务，以及通过测量自发和 阿扑吗啡引起的盘旋。

DESCRIPTION (Investigator's Abstract): The goal of this renewal period will be to study the value of cyclosporine A immunosuppression and basic fibroblast growth factor at improving cell survival and behavioral performance following transplant of fetal mesencephalic dopamine cells in a monkey model of Parkinson's Disease. These studies are a direct extension of the experience of our laboratory in transplanting fetal cells into the MPTP lesioned monkey. Most groups working with transplants in monkeys have found that fetal dopamine cells from early in development (6 to 9 weeks' gestation) can survive in adult brain and produce some behavioral effects. Nonetheless, recovery is not complete, and the survival rate of cells varies considerably between monkeys. The value of immunosuppressant therapy for promoting cell survival and enhancing behavioral improvement is unexplored in monkeys. Basic fibroblast growth factor has been shown in rats to improve fetal mesencephalic dopamine cell survival in tissue culture and in rats to improve fetal neuronal grafts. For these experiments, we will switch from monkeys systemically lesioned with MPTP to monkeys which have been unilaterally lesioned with the neurotoxin MPTP injected into the carotid artery. The reason for this change in MPTP model comes from the difficulty in getting a reproducible and stable Parkinson syndrome of moderate severity in systemically lesioned animals. Transplant success will be evaluated by changes in learned performance in a new motor task we have devised as well as by measurements of spontaneous and apomorphine induced circling.