NMR STUDIES OF HUMAN CEREBRAL METABOLISM IN VIVO

核磁共振波谱（NMR）的新发展技术 ~ 1H、~（13）C和~（31）p的同位素组成将被用来研究正常和无序 大脑代谢的研究 总的来说，这些 非侵入性方法是一种重复测量 功能神经组织中的特定化合物， 活检，但无组织损伤或濒死变化。 测量结果， 它将直接在大脑的各个部分产生， 磷酸肌酸，三磷酸腺苷， 无机磷酸盐、细胞内pH、单酯和二酯库 磷酸、乳酸盐升高、N-乙酰天冬氨酸盐、化合物池 含有胆碱和肌酸以及一些浓缩氨基酸。 的 工作将在新设计的2.3特斯拉牛津/布鲁克光谱仪上进行 具有水质子成像能力;该仪器将是第一个 它的种类。 在对狗进行了一系列简短的实验后， 已建立的光谱仪技术，以更大的机器，标准 将制定人类记录程序，并确定 正常光谱的特征和变化开始。 一项重大努力将 使其定位大脑内的光谱信号源， 结合先进的光谱技术。 痴呆患者 和多发性硬化症将被研究， 灰色和白色物质中神经元和神经胶质的生物化学， 这些细胞类型的比例已被疾病改变。 患者 对于考虑进行颞叶切除术的复杂部分性癫痫发作， 患有中风或有中风风险的患者，以及患有脑肿瘤的患者， 研究以评价NMR可测量量作为结果的预测因子 和对管理层的不信任。

Newly developed techniques in nuclear magnetic resonance spectroscopy (NMR) of 1H, 13C and 31p will be used to investigate normal and disordered cerebral metabolism in living human subjects. Collectively, these non-invasive methods are a means of making repeated measurements of specific compounds in functioning nervous tissue in a manner comparable to biopsy, but without tissue damage or agonal change. The measurements, which will be made directly in various parts of the brain are of the relative concentrations of phosphocreatine, adenosine triphosphate, inorganic phosphate, intracellular pH, pools of mono- and diesters of phosphoric acid, elevated lactate, N-acetylaspartate, pools of compounds containing choline and creatine, and some concentrated amino acids. The work will be done in a newly designed 2.3 Tesla Oxford/Bruker spectrometer with water proton imaging capability; this instrument will be the first of its kind. Following a short series of experiments on dogs to adapt established spectrometer techniques to the larger machine, standard procedures for human recording will be developed and determination of the characteristics and variation of normal spectra begun. A major effort will be made to localize the source of spectral signals within the brain by a combination of advanced spectroscopic techniques. Patients with dementia and multiple sclerosis will then be studied to gain new information about the biochemistry of neurons and glia in gray and white matter in which the proportions of these cell types have been altered by disease. Patients with complex partial seizures being considered for temporal lobectomy, patients with or at risk for stroke, and patients with brain tumors will be studied to evaluate NMR-measureeable quantities as predictors of outcome and adjuncts to management.