CHOLECYSTOKININ IN BRAIN: ITS ROLE IN SATIETY

脑肽在代谢调控中的作用 和行为可能会导致治疗的重要改善， 生长、神经和代谢紊乱，例如肥胖。 拟议 工作涉及两个家族的肽，胆囊收缩素（CCK） 和阿片肽，在控制进食行为。 脑CCK， 是饱腹感的介质，可以起到阻断阿片肽的作用， 刺激进食 然而，看起来，角色取决于 物种 总体目标是利用生物化学、解剖学和行为学 建立CCK和阿片类物质生理参与的技术 肽在控制三个物种的摄食行为， 他们对中枢和外周给药的不同反应 CCK。 实验结果将提供证据1）参与 CCK介导的饱腹感和阿片类药物介导的饥饿感中的特定脑区，2） CCK和阿片肽在脑内特定部位的相互作用 控制摄食行为; 3）种间解剖学差异 这可能解释了它们对CCK和阿片类药物的不同反应 缩氨酸 胆囊收缩素和阿片肽含量及胆囊收缩素受体结合 将比较大鼠、仓鼠和绵羊脑的特定位点 在进食-禁食和肥胖-瘦的条件下。 免疫组织化学将 用于描述和比较CCK细胞体和纤维的关系 对与进食相关的大脑目标中的阿片肽细胞体和纤维产生影响 地区 为了确认通过以下方式建立的不同反应的重要性， 生化和免疫组化检测，CCK和阿片肽 将直接注入大脑的特定部位 将对答复进行衡量。 这些实验的结果将导致 更全面地了解控制进食行为， 也可能是更可预测的控制进食障碍的方法。

Understanding of the roles of brain peptides in the control of metabolism and behavior is likely to lead to important improvements in treatments for growth, neurological, and metabolic disorders, e.g. obesity. The proposed work concerns the roles of two families of peptides, cholecystokinin (CCK) and opiate peptides, in the control of feeding behavior. Brain CCK, which is a mediator of satiety, may act to block effects of opiate peptides, which stimulate feeding. It appears, however, that roles vary depending on species. An overall aim is to use biochemical, anatomical and behavioral techniques to establish the physiological involvement of CCK and opiate peptides in the control of feeding behavior in three species chosen for their differential responses to centrally and peripherally administered CCK. Results of experiments will provide evidence for 1) involvement of specific brain areas in CCK-mediated satiety and opioid-mediated hunger, 2) interactions in specific brain sites between CCK and opiate peptides in controlling feeding behavior, and 3) anatomical differences among species which may account for their differential responses to CCK and opiate peptides. CCK and opiate peptide content and CCK receptor binding in specific loci of the brain of rats, hamsters, and sheep will be compared under fed-fasted and obese-lean conditions. Immunohistochemistry will be used to describe and compare the relationship of CCK cell bodies and fibers to opiate peptide cell bodies and fibers in feeding-associated brain target areas. To confirm the importance of differential responses established by the biochemical and immunohistochemical measures, CCK and opiate peptides will be administered directly into specific brain sites, and feeding responses will be measured. The results of these experiments will lead to a more complete understanding of the controls of feeding behavior and very likely also to more predictable means of controlling feeding disorders.