ELECTROPHYSIOLOGICAL CORRELATES OF VASOPRESSIN RELEASE

目的是了解加压素（也称为抗利尿剂） 激素）分泌是通过控制电 加压素分泌神经元的放电模式 为了实现这一目标， 这些神经元的放电模式与血管加压素的释放相关， 体外系统模型，大鼠视上神经垂体系统，其中 放电模式可以被神经活性化合物改变， 这些神经元的神经递质控制。 具体 目标是1）将组胺和去甲肾上腺素的作用与 加压素的释放，以他们的行动，对突发的电活动， 视上神经分泌神经元; 2）以确定是否不同 这些化合物的同种受体介导不同的作用; 3）确定 这些化合物的相互作用与电状态的 神经分泌神经元诱发电活动的爆发; 4） 简明地定位这些化合物对加压素的作用， 使用细胞内标记和免疫细胞化学的催产素神经元; 5） 开始表征与以下作用相关的膜事件： 这些化合物与细胞内记录;和6），以确定 后叶加压素活性的爆发 与加压素释放的量和时程的关系。 这些目标 将通过采用电生理学，神经药理学， 免疫细胞化学和放射免疫分析。 本文所述的模型系统提供了一种研究 电活动与激素释放的关系。 现在已知 许多非神经元的细胞也表现出电活动 当释放激素时，这些研究所回答的问题可以 可以应用于身体中的许多细胞类型。 加压素 特别是在确定适当的水平衡是非常重要的， 包括人类在内的哺乳动物，及其控制功能障碍导致许多 生理异常，包括尿崩症和慢性 高钠血症

The goal is to understand how vasopressin (also called antidiuretic hormone) secretion is controlled through the control of the electrical firing patterns of vasopressin-secreting neurons. To achieve this goal, firing patterns of these neurons are correlated with vasopressin release in a model in vitro system, the rat supraoptico-neurohypophysial system, where firing patterns can be altered by neuroactive compounds which represent the neurotransmitter control of these neurons in the whole animal. Specific goals are 1) to relate the actions of histamine and noradrenaline on vasopressin release to their actions on bursts of electrical activity in supraoptic neurosecretory neurons; 2) to determine if different isoreceptors of these compounds mediate different actions; 3) to determine the interaction of these compounds with the electrical state of the neurosecretory neuron in evoking bursts of electrical activity; 4) to concisely localize the effects of these compounds on vasopressin and oxytocin neurons using intracellular labelling and immunocytochemistry; 5) to begin characterization of the membrane events related to the action of these compounds with intracellular recordings; and 6) to determine the quantitative relationship between a burst of activity in a vasopressin neuron and the amount and time course of vasopressin release. These aims will be realized by employing electrophysiology, neuropharmacology, immunocytochemistry and radioimmunoassay. The model system described herein provides a means of studying the relationship of electrical activity and hormone release. It is now known that many cells, non-neuronal in nature, also exhibit electrical activity when releasing hormone, and the questions answered by these studies could well be applicable to many cell types in the body. Vasopressin in particular is very important in determining proper water balance in all mammals, including man, and dysfunctions in its control lead to many physiological irregularities, including diabetes insipidus and chronic hypernatremia.