VENTRAL MEDULLA OBLONGATA AND CARDIOVASCULAR CONTROL

延髓腹侧调节交感神经传出活动 心血管系统。含P物质(SP)和 促甲状腺激素释放激素(TRH)从延髓腹侧投射到 脊髓中间外侧细胞柱(IML)(起始点 节前交感神经元)。药理实验表明 P物质介导腹侧的交感兴奋反应 延髓。拟议的实验旨在(1)进一步阐明 SP和TRH在延髓腹侧调节中的作用 研究TRH受体的细胞定位对心血管功能的影响 IML TRH受体激活的交感心血管效应，通过 在体评估延髓腹侧激活对AN指数的影响 SP和TRH周转，并通过确定SP和TRH的影响 区域脊髓和外周血流的类似物；和(2)至 提供更完整的神经解剖学信息。 通过研究终末的起源和细胞位置来研究延髓腹侧 腹侧强直性GABA能神经支配的受体 大鼠延髓SP和TRH神经元的分布及SP和TRH共存的研究 延髓腹侧和终末的非5-羟色胺能神经元。 我们将使用神经解剖学和神经化学技术(神经束 示踪，免疫细胞化学，受体放射自显影，肽周转， 放射免疫分析)和体内神经药理技术(中枢神经系统 心率、血压和局部血液的注射和监测 流)。 这些研究将提供有关化学神经解剖学和 传入(SP、TRH)和传入(GABA)神经元系统的功能作用 腹侧延髓。这一信息对于理解 CNS对心血管系统的控制，并可能使新的设计成为可能 治疗和/或预防心血管疾病的治疗方法和药物 疾病。

The ventral medulla oblongata regulates sympathetic efferent activity to the cardiovascular system. Neurons containing substance P (SP) and thyrotropin releasing hormone (TRH) project from the ventral medulla to the spinal cord intermediolateral cell column (IML) (the site of origin of preganglionic sympathetic neurons). Pharmacologic experiments suggest that SP mediates sympathoexcitatory responses elicited from the ventral medulla. The proposed experiments are intended (1) to further elucidate the role of SP and TRH in the ventral medullary regulation of cardiovascular function by studying the cellular location of TRH receptors and sympathetic cardiovascular effects of IML TRH receptor activation, by assessing in vivo the effects of ventral medullary activation on an index of SP and TRH turnover, and by determining the effects of SP and TRH analogs on regional spinal cord and peripheral blood flow; and (2) to provide more complete information on the chemical neuroanatomy of the ventral medulla by studying the origin, and cellular location of terminals and receptors of the tonically active GABAergic innervation of ventral medullary SP and TRH neurons, and to evaluate SP and TRH coexistence in nonserotonergic neurons in the ventral medulla and terminals in the IML. We will use neuroanatomical and neurochemical techniques (neuronal tract tracing, immunocytochemistry, receptor autoradiography, peptide turnover, radioimmunoassay), and in vivo neuropharmacological techniques (CNS injections and monitering of heart rate, blood pressure, and regional blood flow). These studies will provide information on the chemical neuroanatomy and functional role of efferent (SP, TRH) and afferent (GABA) neuronal systems of the ventral medulla. This information is vital to the understanding of CNS control of the cardiovascular system and may enable the design of new therapeutic approaches and drugs to treat and/or prevent cardiovascular diseases.