ISOLATION OF VERTEBRATE NEURAL CHOLESCYSTOKININ HOMOLOGS

缩胆囊素(CCK)和胃泌素是 神经胃肠肽。CCK是最丰富的 脊椎动物的脑多肽。然而，没有神经功能(S) 为CCK澄清。有证据表明CCK可能参与了 学习、记忆和进食的调节。CCK与 经典的递质，例如多巴胺，可能起到调节 经典发射器的释放。越来越多的证据表明 脊椎动物神经组织中存在未知的CCK相关多肽。一个 彻底了解脑血管紧张素转换酶的生理作用 任何神经肽家族都是识别和表征原发的 该家族中每种多肽的结构。这项提议旨在 脊椎动物脑内CCK相关多肽的鉴定、分离和鉴定 组织。这项建议的近期目标包括：1)色谱学 大鼠脑组织中CCK相关多肽的分离与鉴定 使用CCK样多肽的放射免疫分析，以及2)结构 CCK相关多肽的分析。 CCK相关多肽的纯化将提供氨基酸序列数据 这可以用于进一步的研究。寡核苷酸探针可以是 设计成多肽来分离DNA克隆。特定于序列 抗体可用于定位研究。此信息将 提供机会来解决有关监管和 CCK相关基因的表达、前体加工、多肽位置 在神经组织内的作用，最终，表型和遗传型 CCK相关多肽的特性。完成这些研究 将为研究其重要的功能提供依据 神经肽家族在分子水平上。未来的研究将利用一种 生物化学、组织化学、分子生物学的多学科方法 生物学、神经生物学和生理学研究的问题 调节、功能和表达。拟议中的实验将 增加我们对一个神经肽家族的了解 生理功能。当这个重要的神经肽家族更好的时候 理解，那么也许研究人员就能更好地理解 学习、记忆和进食相关的障碍。

Cholecystokinin (CCK) and gastrin are members of a family of neuralgastrointestinal peptides. CCK is one of the most abundant vertebrate brain peptides. However, no neural function(s) has been elucidated for CCK. Evidence suggests that CCK may be involved in learning, memory and the regulation of feeding. CCK is co-localized with classical transmitters, e.g., dopamine, and may function to regulate the release of classical transmitters. There is increasing evidence that unidentified CCK-related peptides exist in vertebrate neural tissue. A fundamental requirement to thoroughly understand the physiological roles of any neuropeptide family is to identify and characterize the primary structure of each peptide in that family. This proposal is designed to identify, isolate and characterize CCK-related peptides in vertebrate brain tissue. The immediate goals of this proposal include: 1) chromatographic separation and identification of CCK-related peptides in rat brain tissue using radioimmunoassays specific for CCK-like peptides, and 2) structural analyses of CCK-related peptides. Purification of CCK-related peptides will provide amino acid sequence data that can be used for further studies. Oligonucleotide probes can be designed to the peptides to isolate the DNA clones. Sequence-specific antibodies can be produced for localization studies. This information will provide the opportunity to address questions regarding regulation and expression of the CCK-related genes, precursor processing, site of peptide action within neural tissue and eventually, phenotypic and genotypic characteristics of the CCK-related peptides. Completion of these studies will provide the basis for studying the function of an important neuropeptide family at the molecular level. Future studies will utilize a multidisciplinary approach of biochemistry, histochemistry, molecular biology, neurobiology and physiology to investigate questions of regulation, function and expression. The proposed experiments will increase our understanding of a neuropeptide family involved in important physiological functions. When this important neuropeptide family is better understood, then perhaps researchers will be better able to understand learning, memory and eating-related disorders.