MECHANISMS OF FURROW FORMATION DURING CELL DIVISION

该提案要求支持举办一次研讨会， 细胞分裂时沟形成的机制。 本次会议 将于1989年6月8日至11日在荒岛山举行， 缅因州索尔兹伯里湾生物实验室。 它将包括 约25位受邀演讲者+约25位受邀演讲者 审计员。 演讲者将提交手稿发表在 纽约科学院年鉴。 审计员将提交 海报. 细胞分裂不仅包括遗传物质的分裂， 细胞核（有丝分裂），但也分裂的细胞质 （胞质分裂）。 这两个过程都需要一个函数 有丝分裂器 这种结构主要由微管组成 由一个纺锤体和两组星光组成 纺锤体上的染色体分离一直是 以前的会议。 然而，紫菀的生长机制 和/或纺锤体刺激皮质细胞质形成 犁沟和犁沟进行的生化步骤 将细胞压缩成两半， 专题讨论会。 最近的进展是了解 微管和微丝的结构和功能， 基因的一些组成蛋白质，以下 将荧光标记的蛋白质掺入到切割中 沟，用于测量细胞内浓度的变化， Ca 2+，并使用计算机模型来预测 细胞操控 制作这些照片的调查人员 在美国取得了进展，日本、英国和加拿大，许多国家 从没见过 我们对胞质分裂的理解将更加深刻 通过召集这些调查人员参加一个研讨会， 出版一套统一的关于机制的论文。

This proposal requests support for a workshop to discuss the mechanisms of furrow formation during cell division. This meeting will be held during June 8-11, 1989, at the Mount Desert Island Biological Laboratory, Salisbury Cove, Maine. It will consist of approximately 25 invited speakers + approximately 25 invited auditors. Speakers will submit manuscripts to be published in the Annals of the New York Academy of Sciences. Auditors will present posters. Cell division consists not only of division of genetic material in the nucleus (karyokinesis), but also of division of the cytoplasm (cytokinesis). Both of these processes require a functional mitotic apparatus. This structure consists largely of microtubules and is comprised of a spindle and two sets of astral rays. Chromosome separation on the spindle has been the subject of previous meetings. However, the mechanisms by which the asters and/or the spindle stimulate the cortical cytoplasm to form a furrow and the biochemical steps by which that furrow then proceeds to constrict a cell in half have not been discussed in earlier symposia. Recent advances have been made in understanding microtubule and microfilament structure and function, in perturbing the genes for some of the component proteins, for following the incorporation of fluorescently labeled proteins into cleavage furrows, for measuring changes in intracellular concentrations of Ca2+, and for using computer models to predict the consequences of cellular manipulations. The investigators who have made these advances are in the U.S., Japan, England, and Canada, and many have never met. Our understanding of cytokinesis will be sharply stimulated by assembling these investigators for a workshop and publishing a unified set of papers dealing with mechanisms.