MEMBRANE PROTEIN STRUCTURE

这里提出的项目的中心目标是加速 膜蛋白结构研究的进展， 传播关于各种生物物理 已成功应用于膜的技术 蛋白质研究 我们提出的具体方法 信息传播是一个研讨会，我们建议， 1990年10月13日至18日在范德比尔特大学校园举行 大学医学院。 本次研讨会遵循两个 美国膜蛋白结晶学派 分别于1985年12月和1987年10月在科罗拉多博尔德举行， 在阿贡国家实验室， “相互作用的实验和理论方面， 确定蛋白质构象”会议于1989年1月在 国家卫生研究所。 如同第一和第二 美国膜蛋白结晶学派，我们 我选择了一个研讨会的形式，以便与会者可以收到 讨论中的方法的实际经验。 像 在蛋白质构象会议上，然而，我们将给出 研讨会比以往更广泛，包括 技术-除了结晶和衍射 方法-已成功地用于获得 关于膜蛋白结构的信息。 四天的他 讲习班将集中讨论技术组：（1）洗涤剂， 膜蛋白的性质和纯化，（2）膜 蛋白质结晶和衍射方法，（3） 膜蛋白的光谱分析，和（4）实验 膜蛋白的预测方法 二级结构 这四天中的每一天下午 实验室和/或演示，恭维的主题 上午的讲座。 最后一个早晨， 会议将专门深入审查几个 膜蛋白已经被各种研究， 结构方法

The central goal of the project proposed here is the acceleration of progress in structural studies of membrane proteins through the dissemination of information on a variety of biophysical techniques that have been successfully applied to membrane protein studies. The specific method that we propose for the dissemination of information is a workshop which we propose to hold October 13-18, 1990, on the campus of the Vanderbilt University School of Medicine. This workshop follows the two "American Schools on the Crystallization of Membrane Proteins" held in December 1985, in Boulder, Colorado, and in October 1987, at the Argonne National Laboratory, and the conference on "Experimental and Theoretical Aspects of the Interactions the Determine Protein Conformation" held in January 1989, at the National Institution of Health. LIke the first and second American Schools on the Crystallization of Membrane Proteins, we have chosen a workshop format, so that participants can receive hand-on experience with the methods under discussion. Like the conference on Protein Conformation, however, we will give the workshop greater breadth than its predecessors by including techniques-in addition to crystallization and diffraction methods-that have been successfully used for obtaining information about membrane protein structure. Four days of he workshop will focus on clusters of techniques: (1) detergents, membrane protein properties and purification, (2) membrane protein crystallization and diffraction methods, (3) spectroscopic analyses of membrane proteins, and (4) experimental and predictive methods for investigating membrane protein secondary structure. Each of those four days will have afternoon laboratories and/or demonstrations that compliment the subject matter of the morning lecture. The final morning of the conference will be devoted to in-depth reviews of several membrane proteins which have been studied by a variety of structural methods.