COORDINATION CHEMISTRY OF VANADIUM IN BIOLOGY SYSTEM

描述(改编自《调查员摘要》)： 钒在生物系统中的地位已经得到了很好的证实。阿玛瓦丁来自 蘑菇A.Muscaria，血细胞中V(III)-金黄色系统 几种海藻的被毛类、溴过氧化物酶和 棕色固氮菌含钒固氮酶突出 举个例子。现在也很清楚，钒具有许多重要的 环境、毒理和生理影响。尽管它的 存在，几乎什么都不知道，关于结构，功能 或在任何这些系统中的钒的化学。在这方面的实验 提案旨在帮助勾勒出可能的结构、氧化状态 和钒的连接，从而产生了物理化学性质 这些生物分子。准确的结构信息至关重要 对理解钒在生物学中的作用的重要性。一个 定义明确的小分子模型与生物分子的比较 它们本身就是实现这一目标的一种可行手段。这个 调查人员建议使用模型化合物来帮助调查两个 特别感兴趣的领域：1)行动的结构和机制 第一，真正的香草酸酶，“溴过氧化物酶”；2)其作用机制 被毛状物中钒的积累及其生物学功能。特定的 要解决的问题/目标包括： 1)对于溴过氧化物酶：生产准确的小分子模拟物 对于活动站点，它将提供有关其结构和 作用以及对其作用机制的调查 酵素。这些研究将涉及新钒的合成。 络合物及其完全表征。这样的描述将会 包括UV-Vis分光光度法、核磁共振(1H、13C和51V适当)， EPR，磁性测量，电化学，适当时，单项 晶体X射线衍射仪。 2)对于衣酸盐：了解钒与金属间的相互作用 并确定还原/积累的机制 与其吸收有关的过程。从这些研究中，调查人员还 希望能够了解钒在人体内的生物学功能。 这些有机体。这些研究将涉及共振拉曼和磁学 模型化合物和实际被膜血液的敏感性研究。 这项研究项目的圆满结束将为 为提高研究人员对钒的作用的了解奠定基础 在许多生物系统中。

DESCRIPTION (Adapted from the Investigator's Abstract): The presence of vanadium in biological systems is well established. Amavadine from the mushroom a.muscaria, the V(III)-tunichrome system in the blood cells of tunicates, bromoperoxidases from several species of marine algae and a vanadium containing nitrogenase from azotobacter vinelandii are salient examples. It is also now clear that vanadium has a number of important environmental, toxicological and physiological effects. Despite its presence, virtually nothing whatever is known about the structure, function or chemistry of vanadium in any of these systems. Experiments in this proposal are designed to help outline possible structures, oxidation states and ligation of vanadium that give rise to the physicochemical properties of these biomolecules. Accurate structural information is of paramount importance to an understanding of the role of vanadium in biology. A comparison between well-defined small molecule models and the biomolecules themselves represents one viable means of achieving this goal. The investigators propose to use model compounds to help investigate two particular areas of interest: 1) the structure and mechanism of action of first true vanadoenzyme, "bromoperoxidase" and 2) the mechanism of accumulation, and biological function of, vanadium in tunicates. Specific questions/goals to be addressed include: 1) For Bromoperoxidase: The production of accurate small molecule mimics for the active site which will give information about its structure and function along with investigations into the mechanism of action of the enzyme. These investigations will involve the synthesis of new vanadium complexes and their complete characterization. Such characterization will include UV-vis spectro-photometry, NMR (1H, 13C and 51V as appropriate), EPR, magnetic measurements, electrochemistry and, when appropriate, single crystal X-ray diffraction. 2) For Tunicates: To understand the interaction between vanadium and the tunichromes and to determine the mechanism of the reduction/accumulation process involved in its uptake. From these studies the investigators also hope to be able to understand the biological function of the vanadium in these organisms. These studies will involve resonance Raman and magnetic susceptibility studies on both model compounds and actual tunicate blood. The successful conclusion of this research project will provide a sound basis to increase the investigators understanding of the role of vanadium in many biological systems.